Revvity biosciences has stoped making the Revvity Health Sciences Inc RediJect Coelenterazine h Bioluminescent Substrate. Has anyone found or used a good substitute that has a short half-life for in vivo assays?
You can purchase CTZ-h from Nanolight and develop your own stock for injections: e.g. in a 50/50 mix of "Ethanol /propylene glycol" or in 30 %w/v SBE7-β-CD (sulfobutylether-β-cyclodextrin; Captisol) (https://www.protocols.io/view/chemiluminescence-of-coelenterazine-catalyzed-by-c-5qpvoy7n9g4o/v3/materials).
If you are using Renilla luciferase, then Rluc8SG luciferase in living cells has only slightly lower activity with bis-CTZ (bis-CTZ is much more cell permeable than CTZ-h), so you can try bis-coelenterazine as well (it is available from multiple suppliers).
For Nanoluc luciferase, bis-CTZ is generally better than furimazine.
Also, there is a new SSluc luciferase (based on Nanoluc) which is even more active: https://www.linkedin.com/posts/mkoksharov_the-first-proper-luciferase-based-calcium-activity-7314657803254521856-J_pN
Generally, all CTZ-related substrates are very short-lived in the bloodstream: 1) they get oxidized relatively fast (7-20 min half-time) by serum albumin (accompanied by some luminescence); 2) removed by the renal system and by general degrading enzymes; 3) cytoplasm of cells absorbs them a lot, so they should have a very large volume distribution.
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