If I wanted to do cell viability using mts assay, the treatment need to be diluted with complete media or basal media? What happen if I used complete media to dilute the treatmemt and do cell viability test?
Ewelina Barbara Honkisz-Orzechowska
Dear Melonney,
I recommend diluting in complete media (appropriate culture medium + 10% FBS or other serum). Reducing the concentration of serum during the treatment is an additional factor that might influence cell viability. Remember also what the diluent of your treatment is. If it is DMSO, do not exceed the final concentration of DMSO of 0.1%. Good luck!
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