I used both lipofectamine RNA iMax and electroporation method and always gene expression level in negative control group is 40% lower than untreated group.
What did you measure? The level of the transcript or the protein? They don't always correlate!
How abundant is your target transcript on your cells? Is it too low and what you detected were noises?
I measure level of gene expression.
Gene expression can be level of RNA or level of protein, it is a very vague word.
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
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During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
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Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
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A molecule shows the -11.6 kcal/mol and second one -8.0 kcal/mol so why molecule (A) revealed higher binding score. Please explain major factor involve in it.
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Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
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I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
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Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
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