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Hi, I am looking for a resource that can show me the dietary fiber composition of certain feed ingredients in animal feed. For example I want to know the main types of hemicellulose fibers...
08 December 2023 6,331 0 View
I am trying to establish a good protocol to stain neurons in whole fish brain. I know that tissue thickness affects the antibody incubation time, but I was wondering whether tissue clearing also...
06 December 2023 1,715 0 View
I am testing a new antibody (CD94) to stain for NK cells in the brain. However, when I test it in tonsil tissue, it stains basically every cell in the germinal center, so I did another test with...
05 December 2023 6,334 0 View
I have attempted numerous times to achieve a nitrogen content of 26% in calcium ammonium nitrate, but consistently obtained a value of 22.93%. Can anyone provide assistance on achieving the...
05 December 2023 5,823 5 View
I really want to know what is the best timing to inject tamoxifen to induce PDAC. My preliminary experiments is not enough to give a suggestion. I have read some papers and they have different...
05 December 2023 9,248 0 View
The expiration date of my PEI transfection reagent is out and I do not know how to check it. I only use siRNA in my work and I can't figure out what I'm doing wrong or what's wrong with PEi. Can I...
05 December 2023 4,759 2 View
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05 December 2023 5,779 4 View
Hi everyone, I aim to isolate MSCs from the bone marrow of the mouse. I put the bone marrow cells into the cell culture flask containing DMEM low glucose+BFGF+vit C. I know that now I have lots of...
04 December 2023 2,584 3 View
Can you please send me the .cif file of Manganese ferrite (MnFe2O4) cubic spinel ferrite ?
04 December 2023 9,663 3 View
Gel electrophoresis, recombinant protein, expression in bacteria, molecular biology
30 November 2023 2,051 6 View
Animal models for in vivo studies of cancer, according to my search, include induced and spontaneous ones,in which the induced ones can be by physical, chemical, and trasplantation based. But...
30 November 2023 8,096 1 View
Hi, I've been having problems working with small organoids (around 100μm in diameter) for immunostaining. Whether I'm passaging them or fixing them for imaging, I consistently face the issue of...
30 November 2023 6,470 3 View
Is it possible to measure the amount of thermogenesis (turning white fat into brown) using its markers LIKE UCP-1 in the blood(PLASMA)?
30 November 2023 7,658 1 View
Information about, how to download and use them for identification of fungus is needed.
29 November 2023 7,721 1 View
What is the difference between the following species: Solea solea and Solea vulgaris from the taxonomical point of view?
29 November 2023 9,484 4 View
We are running western blot for checking the ag-ab interactions.our proteins are from molecular weight 250 to 7 kda.But we are facing some technical issues with the transfer of proteins(i'm not...
29 November 2023 1,386 2 View
It was a large scale culture of a single strain. Only 1 of the flasks showed cell death while others just grew fine. What could cause contamination that contribute to cell death, given that the...
29 November 2023 1,920 1 View
I want to know how i can use SEM and TEM micrographs to discuss mesoporous and microporous nature of materials
29 November 2023 353 1 View
I have sorted 8000 cells into 2mL of media and then transferred them into wells for further processing but my cells have not been able to grow
29 November 2023 4,817 3 View
For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars
28 November 2023 5,704 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
28 November 2023 7,954 1 View
I am using premade gels (4-12%) and using Running Buffer Tris Glycine Buffer and Licor SDS loading buffer
27 November 2023 8,964 0 View
How can I get a procedure for extraction pleurotus oyster polysaccharide by water extraction?
26 November 2023 6,869 2 View
Hello, everyone. Now I am tring to transfect the NK92 cells with lentivirus, athough I failed many times. If you have experience in this experiment, please teach me how to succeed. Thank you. Yongyu
26 November 2023 1,817 0 View