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Questions related to Tissue
I'm looking to the best way to approach Receptor Occupancy (RO) determination in peripheral tissue for a monoclonal antibody targeting a cell surface protein in a peripheral tissue. For...
25 October 2021 8,201 0 View
Hi All, I am going to run BDNF ELISA in mouse brain tissue (prefrontal cortex and hippocampus). Can any one suggest a good lysis buffer and in what ratio should the protease inhibitor be mixed...
23 October 2021 613 1 View
We are trying to measure cathepsin B activity in the cell supernatants or broncho-alveolar lavage fluid. Is there a good assay that can measure the activity?
22 October 2021 3,963 1 View
I have recently discovered that a good chunk of my brain tissue has holes in it. So much so that it almost looks like swiss cheese. We have had this issue in the past and we concluded that it was...
21 October 2021 4,396 3 View
I am doing IHC on whole mount retina with a secondary antibody conjugated to AlexaFluor 488. I had a weak signal and I wonder if I could wash my retina in PBS-Triton or in a stripping buffer to...
21 October 2021 9,991 1 View
I'm new on medical science and I learned from my teachers that there are 4 specialized myocardium conducts on the right atrium that make the internodal conduction. I did an fast review on...
20 October 2021 6,651 3 View
I have an endocast that I am trying to measure the volume of the olfactory bulbs from. I have the data segmented in Avizo, but unfortunately because of the way the specimen was scanned (it was a...
20 October 2021 1,364 1 View
Can anyone recommend a protocol for preparation of tissue samples for FTIR analysis? I have frozen tissue samples that I would like to analyze by FTIR but I don't know what would be the best way...
20 October 2021 1,739 0 View
We would like to do a Caco-2 assay of 5 compounds, and we'll have more compounds to do next year. So, is this possible that the two batches of results differs a lot? Or the results will be stable?...
20 October 2021 1,357 1 View
DNeasy Plant Kits provide a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed using the DNeasy Plant Mini Kit or up to 1 g of tissue using the...
19 October 2021 2,876 3 View
varroa destructor honey bee ectoparasite
19 October 2021 5,275 3 View
To preserve eDNA filters (e.g., Sterivex filters) for eDNA studies of metazoans, the two most common preservation methods of these filters are, after water filtration, either: - frozen at -80°C -...
18 October 2021 9,716 0 View
Hello all Professors What are the kits used in 16S rRNA Metagenomic sequencing (MiSeq Illumina) after the DNA extraction from human tissues (biopsy)? Thank you in advance
17 October 2021 8,689 2 View
Samples were homogenized with a mortar and pestle and then passed through a syringe but at the first centrifuge step the sample is getting stuck - we can't see anything on the membrane and only...
14 October 2021 8,765 3 View
Hi, could you teach me if anyone has experience in detection of Reactive oxygen species? I would like to detect ROS in the cytosol and in the mitochondria separately. Mitosox can detect...
14 October 2021 8,348 6 View
Hi everyone, it's now several weeks that I have problems for the sequencing of mouse samples with the MinION device (from Oxford Nanopore Technologies). For that, I extracted gDNA from mouse...
14 October 2021 8,279 0 View
I'm planning to collect blood in EDTA tubes in a remote area in which I don't have access to immediate staining of cells. I'll be collecting samples for 2 weeks then transporting cells to the lab...
13 October 2021 4,040 2 View
Our long standing understanding for mammalian ovary is that it has fixed number of germ cells and duty of the ovary is to ovulate high quality ovum for successful fertilization and early embryonic...
12 October 2021 927 8 View
I am currently amplifying a 2kb fragment from genomic DNA from mouse liver tissue. I am not sure if the DNA sample is of good quality or maybe it is even broken into fragments.I learned that I can...
11 October 2021 3,563 1 View
Hello, In our ASC (adipose derived stem cells) culture we observed different morphology than usual. What could it be ? Rounded shape cells instead of typical fibroblast-like cells? No...
11 October 2021 1,371 5 View
Hello everyone, My colleague and I are working on creating a protocol for examining tendon mechanical properties, but are having trouble finding a description of how tendon fibrils are isolated....
10 October 2021 9,173 3 View
I'm looking for a good oligodendrocyte antibody to do some double stain with BrdU in rat and mouse brain tissue. What do people recommend?
08 October 2021 405 3 View
I am sectioning brain tissue. During this process sometimes the tissue separates from the OCT or there will be bubbles in brain tissue folds that make the section wrinkle more. These can hinder...
08 October 2021 3,284 2 View
For isolation of mitochondria from rat hippocampus, chilled isolation medium (0.25 M sucrose containing 10 mM Tris–HCL, pH 7.4, 1 mM EDTA, and BSA 250 lg/mL) is made. Can we make this solution...
08 October 2021 5,213 7 View