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Questions related to Tissue
Hi all, I'm working with transfected and immunostained cells (fixed) that have been imaged by confocal microscopy. In one of the cells, I observed a striking morphology — several large...
26 June 2025 2,839 1 View
This is for a literature review
25 June 2025 3,392 1 View
Hi, weird question about splicing. (I am not a splicing expert tbh it kind of baffles me) if we have an intron 1 mutation that destroys a splice site causing an intron 1 retention, could that...
25 June 2025 3,046 2 View
How could AI improve sports and exercise?
24 June 2025 3,959 3 View
2636B was used to get the results but it seems the crocodile probes are too hard and the soft probes don't grasp the film properly.
24 June 2025 6,644 6 View
I did an MTT assay test, then gave the MTT reagent to my cells, then during the observation after giving the MTT reagent, purple granules were seen as in the picture. Is it true that formazan...
23 June 2025 901 3 View
How to measure leadership competence and its impact on directing work teams and developing performance in sports institutions
23 June 2025 8,492 7 View
Hi, I am a PhD Scholar. I am in need of valid and reliable scale of HRM System Maturity. But i found a one item scale. Is it feasible for PhD Study to measure a construct through only one item...
18 June 2025 8,114 4 View
Dear All, I try to print CO2 and H2O adsorption number and energy in LAMMPS using GM. But I cannot get any results in output. I add the relevant LAMMPS files. I do not know what I miss it. In...
18 June 2025 4,502 5 View
Thank to anyone spear their attetnion to my question. I am working on flurence fluorescent immunostaning staining CSF1R in both paraffin-fixed and PFA-fixed human glioma samples. I am faced with...
11 June 2025 3,012 2 View
In my recent submissions of qualitative research papers to international journals, I have grounded my sampling rationale on the concept of "information power" as proposed by Malterud et al....
11 June 2025 1,576 1 View
Hello everyone, At our lab, we currently perform manual co-extraction of DNA and RNA from FNA (fine-needle aspiration) slides using Trizol reagent and this protocol is effective in preserving...
10 June 2025 7,416 1 View
Hi all, We are currently working on extracting microbial DNA from clinical blood samples (from BacT/ALERT bottles) for downstream NGS applications. We’re using a commercial kit for microbial DNA...
10 June 2025 9,359 2 View
I performed phage genome extraction using phenol-chloroform-isoamyl alcohol (PCI). Prior to extraction, I treated the sample with DNase and RNase to remove any non-phage nucleic acids. After PCI...
10 June 2025 7,545 2 View
Hi everyone! I’ve been using ResearchGate for a while now, mostly as a quiet reader—exploring papers, following topics, and learning from what others share. But recently, I’ve started posting...
09 June 2025 4,960 2 View
Hi, I have recently thawed a 3T3-J2 cells from our stock. Initially the cells look like the top image and as I passaged, it became more elongated and "more" fibroblastic/ spindle-like compared to...
09 June 2025 6,447 6 View
Can you recommend any literature on the differences between pharmacological inhibition and the knockout model, in terms of advantages and disadvantages? Thank you in advance. Best Regards
08 June 2025 4,272 3 View
Hello, everyone I have some experimental problem about ligation. I use CloneJET PCR Cloning Kit-blunt ends (Thermo Fisher Scientific) to ligate my target gene which expected band is 1...
30 May 2025 9,759 3 View
Tribromoethyl alcohol (Cat.No.: HY-B1372) is a commonly used animal anesthetic in laboratories: In addition to the usual protection of wearing gloves and fume hoods, are there any special...
29 May 2025 2,587 1 View
Local crops and livestock are hardy but producing less. How can we manage this controversy to become self-sufficient in food production?
28 May 2025 1,260 2 View
I am doing a research with anaplasma sp. And I have infected my cell line with anaplasma possitive blood RBC and after 1-2 days of incubation an odor is produced in the incubator.
28 May 2025 4,733 0 View
Hello everyone, I typically obtain good results using the commercial silica column-based DNA extraction kit from QIAGEN (dried mushroom specimens and pure cultures). However, I’ve recently...
26 May 2025 1,145 1 View
Hi all, I’m studying how heatwaves impact the daily activity of older adults. Does high temperature reduce their mobility or change their exercise habits? Any studies or experiences on this topic...
26 May 2025 9,578 2 View
Hi everyone. I am interested in removing efficiently from media (DMEM high glucose w/o phenol red+glutamine+P/S) conditioned by commercially available primary human adipose tissue mesenchymal stem...
24 May 2025 2,126 2 View