Contact experts in Tissue to get answers
1,122 views 2,268 posts
Questions related to Tissue
So, I want to perform a hydroxyproline assay with my collagen samples. I ordered collagen from bovine achiles tendon as standard but can not dissolve it. I did it in TES buffer as the sigma...
14 March 2022 3,469 3 View
why do we use negative selection marker genes in transgenic plants as they won't survive? and can we grow transgenic cells by negative selection?
11 March 2022 9,940 8 View
I want to work on the effect of nitrate fertilizer on toad (Bufo bufo)
09 March 2022 3,325 5 View
Hi I have a challenge with a somatic mutation in the beta cells. I think I know the disease-causing gene GCK due to the histological picture, but how can detect the mutation?. I have tried to use...
09 March 2022 3,410 2 View
I need using cryostat with NO fixing embryos. Any suggestion for getting more "robust" sample? I have some tissue problem after sectioning... Thank you so much!
09 March 2022 4,271 0 View
Currently I use Leica BOND Plus slides to mount 5 micon thick FFPE sections of mouse knee joints. These have been fixed in PFA and decalcified in EDTA by experienced researchers, and have been...
08 March 2022 8,765 2 View
For THP-1 differentiation we use reconstituted PMA at 1mg/ml. Our final concentration added to cells is 50ng/ml. Since final working concentration & volume required is low, we usually make...
04 March 2022 4,628 3 View
Hi All, I was recently isolating proteins in interstitial fluid from tissue cells. To do that, I firstly used PBS (+proteinase inhibitor) to homogenize the tissue. And I spin the cells down at...
02 March 2022 5,248 3 View
Hy everyone, The investigations for the biological effects of microwaves are the major concern because modern life is surrounded by electromagnetic waves which led us to frequent exposure every...
01 March 2022 6,448 7 View
I'd like to flash freeze PFA-fixed mouse brains in OCT. Usually, this is done by flash freezing with liquid nitrogen, or dry ice. I was just wondering if it would be enough to transfer the...
01 March 2022 9,951 2 View
I am monitoring the expression of mitochondrial membrane-bound proteins in European pear peel tissue via quantitative fluorescent western blot analysis. I am normalizing on membrane with...
01 March 2022 8,065 1 View
I have done Double disc synergy test of bacterial clones in presence and absence of EDTA. The plates show positive results for the presence of MBLs but still susceptible for carbapenems and...
28 February 2022 4,670 2 View
Hello everyone! We have fibroblasts isolated from hepatocellular carcinoma tissue samples. We are planning transcriptomics. However, I am not sure if it is doable since there are only 2500-3000...
25 February 2022 9,160 1 View
My work informed us of a 6 hours power cut to the whole building in a couple of months time in April. We have thin tissue cryosections (for immunofluorescence staining) and aliquots of primary...
25 February 2022 4,415 3 View
I have a fairly simple question: Is it better to consistently use tissue from a single hemisphere for one type of analysis? For example, if you are doing both immunostaining and RNAscope, should...
24 February 2022 4,979 2 View
Hi, anyone know what the heck are those square things in my ultracentrifuged medium?
23 February 2022 4,332 5 View
i want to use tamoxifen diet to induce CreER-DTA mouse deplete specific cells for 3 months, 1、Are there any difference between pure tamoxifen and tamoxifen citrate? 2、Does 80mg per kg more...
23 February 2022 1,717 0 View
gMSCs were seeded on PCL/HAp scaffolds to see their adhesion and distribution properties on it. But even after 2 weeks of incubation, the cells are forming globular morphology rather than flat or...
22 February 2022 7,291 3 View
Hello! I tried to block my fixed cells on 10% FBS and 5% NGS (Normal Goat Serum) in PBS1X for 1h at RT and then incubate with a primary Ab anti-Mouse (4 °C ON in 10% of the blocking solution) and...
22 February 2022 5,554 6 View
Dear researchers, Is there a simple protocol of tissue gravimetry method to measure cerebral edema than that of Marmarou et al, 1978? And is it possible to measure it by the method of Karl...
20 February 2022 6,428 2 View
Tissue culture cells, laboratory animals and embryonated chicken eggs were known to be the methods for viral isolation. Specific pathogen free-embryonated chicken eggs (SPF- ECE) became the most...
20 February 2022 3,392 11 View
Hello friends, While I was reviewing research related to autophagy activation, I noticed that there are two types of LC3 antibodies used to detect the activation of autophagy. Those two types...
19 February 2022 8,611 9 View
Could anyone suggest to me a good protocol to detect Caspase-3 and cleaved Caspase-3? Need to know what percentage of gel to use? Running and transferring conditions?
16 February 2022 4,938 3 View
Hello everyone, My lab is currently looking for a hamster brain atlas and cannot find anything. If anyone has a good source to one, I'd appreciate it. Thank you.
16 February 2022 6,534 2 View