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Questions related to Tissue
Hello, I am currently extracting DNA from formalin-fixed (25 mg) and paraffin-embedded (FFPE) tissues(15 sections of 8 micrometers each), but I am encountering some issues. Since I do not have...
28 March 2024 4,016 5 View
Plant genetics
26 March 2024 1,085 1 View
Dose and time of upregulation of CD86 and CD80 in RAW264.7 cells using LPS/IFNgamma？
25 March 2024 5,556 1 View
I am using these product from ATCC (Primary Uterine Fibroblast Cells; Normal, Human (HUF) ATCC Cat. No. PCS-460-010 ) with their recommended media and with Growth Kit-Low Serum aacording to their...
25 March 2024 255 3 View
i am working on Trichoderma spp.
25 March 2024 8,318 3 View
I am preparing a book discussing the neurological and bio-psychological differences in brain structures that are different in males and females and how that impacts on behavior. Are we...
24 March 2024 2,800 2 View
Dear Research Community, I am writing to seek insights and advice regarding an issue encountered with the revival of the MDAMB231 cell line. Approximately one month ago, I preserved the MDAMB231...
24 March 2024 8,757 2 View
I am a postgraduate research student working on cancer biology in India. I need Danio rerio (Zebrafish) for this study of mine at the earliest. Can someone please provide me with contacts for...
22 March 2024 6,363 1 View
My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...
22 March 2024 8,278 1 View
I am writing a research paper on resolving workplace conflict. I need help writing problem statement and proposal and any suggestions for references.
20 March 2024 8,967 3 View
I'm planning to use the SequelPrep Normalisation plate kit to purify and normalise my PCR amplicons for library construction. According to the manual, the kit is able to clean away proteins and...
20 March 2024 7,118 0 View
I have done Impedance analysis of a pellet with silver pasted electrodes in Hioki3536 LCR meter, but my data is so messy and Zig zag, in nyquist plot. I have make the Pellet again an the data is...
19 March 2024 6,867 2 View
Dear researchers, a variability in the taxonomy classification of microbial communities when using different primer pairs (e.g. for 16S rDNA) is commonly known. However, the mismatches to these...
18 March 2024 8,161 1 View
I prepared MPP+ iodide dissolved in water and its stock concentration is 10M. I stored them at -20℃. Then I treated MPP+ 20mM, 10mM, 5mM, 2.5mM for 24H in SH-SY5Y cells. But they don't seem like...
17 March 2024 7,859 1 View
Has anyone used the second derivative of the bacterial suspension density growth curve to compare the growth of bacterial cultures in a bioreactor?
15 March 2024 5,066 2 View
Specifically address the role of the pure LiZnBO3crystalline phase formation after 8 hours of heat treatment in achieving the enhanced specific capacitance and energy density. How does the...
15 March 2024 7,630 2 View
I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...
13 March 2024 9,370 3 View
I usually use PEG-200 and PEG-300 that are in liquid form. Recently I received PEG-100 from a company that usually do not make PEG-100, and made it once specially for us. It is not in liquid...
13 March 2024 1,789 5 View
I would be very thankful, if someone could advise about literature ans studies. Mnay Thanks in advance Best regards Günter
12 March 2024 8,955 0 View
I found the optimal antibody dilution and incubation time to stain cells of fish brain sections (50μm thick). However I need to stain those cells in thicker brain sections and I was wondering what...
12 March 2024 646 1 View
I am currently working on a CRISPR Cas9 project that involves preparing DH5a competent cells for bacterial transformation. I conducted heat-shock transformations using PUC19 as a positive control...
10 March 2024 8,961 1 View
I want to develop a nanoparticle-based drug delivery platform for burst/instant release of any drug. Kindly suggest ways to do that? What type of release mechanism can be adopted? Usually, applied...
09 March 2024 5,316 1 View
Dear colleagues, We have recently optimized a TDS-GC-MS method for VOCs (SVOCs) analysis. (Gerstel + Agilent). A high-temperature column with mid-polarity is chosen for a better resolution...
09 March 2024 2,738 5 View
I want to know more about U deposits.
09 March 2024 5,367 0 View