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Questions related to Tissue
Hello everyone, I would like to do FISH for SV40 on our cell model, but I can't find a probe from different suppliers (and even in some articles). Would you have a probe reference for me? Or do...
07 October 2021 5,189 4 View
I wanted to inquire about whether anybody had experience with sampling frozen human spinal cord blocks? I am interested in conducting the following upstream of single-cell protocols: 1....
05 October 2021 8,983 1 View
Stability of RNA samples is very low. I would like to know the best company that can transfer my RNA samples with out loss of their stability.
05 October 2021 9,140 1 View
I am isolating whole protein from a fish tissue, now i want to know, using protease inhibitor is it must ? Or can proceed using PMSF only ?
04 October 2021 4,583 3 View
Dear colleagues, I would like to cryosect aortic plus perivascular adipose tissue from obese mice. Unfortunately, at -23°C, with a new knife, embedded in Tissue Tek and very careful handling, the...
03 October 2021 792 2 View
Can anyone recommend a FOXP2 antibody for immunohistochemistry or immunofluorescence for paraffin-embedded human brain tissue? Thank you!
28 September 2021 8,972 3 View
I have perfused and frozen rat brain tissue (from a new transgenic line) that I have cryosectioned onto slides in order to characterise a number of different cell types. One thing I would like to...
27 September 2021 1,609 1 View
I am trying to fixate about 6 heart valves during the course of the day. While I plan on making a fresh formalin solution ever day I have experiments, is it advisable to change the my fixative...
27 September 2021 522 5 View
I have isolated mitochondria from tissue. I would like to check them by fluorescence imaging. Can I use Mitored dye for these isolated mitochondria?
26 September 2021 5,326 2 View
I would like to measure the TTC stained mouse/Rat stroke brain infarct in a square millimeter area using ImageJ.
26 September 2021 3,463 5 View
Could you confirm that the photographed specimen is Magnisudis prionosa?
24 September 2021 2,230 1 View
HI, I used FACS (BD Aria III) for cell sorting. I tested FACS cell sorting for in vivo cells isolation. I used Raw264.7 cells with stained anti-CD11b-PE and anti-CD45-APC. Cells were well...
24 September 2021 4,972 2 View
I currently use the following protocol for fixation of Drosophila melanogaster tissue (chest): -1 h in PFA 4% - washing twice in PB 0.1 M for 15 minutes each - 2 h in sucrose 15% - overnight in...
23 September 2021 181 2 View
Hi. What is the best micro-homogenizer for small tissues? (suitable for use in different types of tissue (from soft to bone tissue) I look forward to your reply...
22 September 2021 5,866 2 View
I am working on a grant application and looking for a practical recommendation regarding oxygen sensors for cell culture media and tissue (tissue engineering constructs). I don't specifically need...
21 September 2021 8,250 8 View
Hello everyone, I would like to purchase a hypoxic chamber to study the effect of magnetic stimulation on hypoxic N2A cells. The cells will be exposed to hypoxia (1% oxygen) only for 3 hours...
21 September 2021 4,523 0 View
Hi, I am looking for an antibody that can specifically detect ferroptosis in kidney tissue. Does anyone have experience with transferrin receptor or Anti-MDA antibodies in kidney tissue? Thank...
20 September 2021 3,428 2 View
For the central nerve system, the use of c-Fos as a marker of activity in certain regions of the brain are widely used. My question is, are there similar markers in the peripheral system? More...
20 September 2021 4,974 1 View
I am having trouble finding the HBL-100 cell line information. Could someone help me?
16 September 2021 7,919 3 View
I am looking for a protocol to determine the metallothioneins (MTs) in mussels, using spectrophotometry in microplates. I homogenized the mussels' tissue with phosphate buffer (pH 7.8 0,1M) to...
15 September 2021 6,450 2 View
Hello! Does anyone have any experience using a higher amount of fresh-frozen tissue (>20 mg of tissue in RNAlater or >30 mg frozen tissue without stabilizing reagent) for DNA/RNA/miRNA...
15 September 2021 7,118 2 View
I am doing IHC and I did not get any good signal on the positive control tissue. I am doing a few more tests, but was looking over the spec sheet and noticed it does say “binds selectively to...
14 September 2021 9,756 1 View
Hi I am testing out the use of Hartman's fixative on 4dpf zebrafish comparing it to NBF. Has anyone done this before? I am concerned how it is impacting the tissue as it is fixed compared to the...
13 September 2021 995 2 View
I am using SigmaAldrich complete EDTA free protease inhibitor cocktail (catalogue no 05056489001) during cell lysis. Can anyone provide the exact composition of this cocktail? I am unable to find...
09 September 2021 8,358 2 View