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Questions related to Tissue
Dear Experts... Your Suggestions are needed to make Plant Tissue Culture (MS) medium. Could Ammonium Sulphate [(NH4)2SO4] be a substitute for Ammonium Nitrate (NH4NO3) in MS Medium? If so..!...
25 May 2022 8,427 11 View
I want to analyze propionic acid from rat serum samples by HPLC. Do you have an easy-to-apply suggestion for the extraction step? Thanks.
24 May 2022 4,697 1 View
From where we can procure Ammonium Nitrate (NH₄NO₃) for Plant Tissue Culture medium preparation? Please suggest some leads
20 May 2022 3,612 4 View
Hi Rg community members, I have been having heck of a trouble to do paraffin sections of adult zebrafish... -I Had decalcified with two methods, and still my tissue shreds when going under the...
18 May 2022 3,161 0 View
The result of the analyzed honey electric conductivity was 0.89 mS/cm and ash content was 0.64 g/100g. However, the standard electric conductivity of honey is < 0.80 mS/cm and ash content is...
14 May 2022 3,498 2 View
There is a antioxidant defense system in cell. Have any signalling pathway or other things can influence ROS level in a extracelluar way?
11 May 2022 8,021 1 View
Hi everyone, I am looking for a human skeletal muscle cell line which however requires differentiation times of 7-10 days and which can grow in horse serum. Could someone recommend me an easily...
07 May 2022 7,613 2 View
In affected patients with endotracheal intubation. Good nigth
05 May 2022 9,080 2 View
Dear all, I may have a silly question, when i was trying to perform clustering by FlowSOM or Phenograph, two plugins from flowjo, nothing came out. Did i set something wrong? I am using M1 mac,...
30 April 2022 1,701 3 View
Dear all, I'm facing a protein-binding issue in my final product. I currently add BSA to protect the protein and avoid the binding to the polypropylene tube. However, I can't do any UV280...
29 April 2022 1,388 5 View
I want to measure the depth of the tissue using dielectric constant/permittivity. In the attached picture different color represents different material. I want to measure the depth of the first...
27 April 2022 6,998 0 View
Is it necessary to dechorionate the early stage zebrafish embryos (
13 April 2022 7,764 4 View
How would one associate a single phenotype factor (binary presence / absence of trait) to SNPs in Tassel (v5.2.58) GUI? I generated VCF file using GATK and am able to import the VCF and...
08 April 2022 5,859 3 View
Hello, I have problems with the data from one study in a meta-analysis. When I manually enter the mean, standard deviation and total data for my two groups into the RevMan software, the...
05 April 2022 2,029 4 View
I want to incubate EVs with cells and then see them in confocal, I think to use covers inside 24-well plates, which minimum volume with EVs can be placed in these wells x 24 h???. And is correct...
05 April 2022 6,467 0 View
Hi, I want to analyse cells by microscopy but after FACS cell sorting. Therefore cells will be fixed (PFA or saponin) before being applied to microscopy slides therefore adhesion to glass slides...
01 April 2022 1,416 0 View
Dears, Even by poultry species standards chickens are more susceptible to diseases. What would be the case?
30 March 2022 3,831 19 View
I will be performing ELISA on patient blood that was discovered to be stored at 4C for about 5 weeks. I was unable to attain standard separation upon centrifugation at 2500rpm for 10min. What...
29 March 2022 2,177 1 View
I want to find out potential mechanisms of the effects of a certain gene on recombinant protein productivity . So proteomics analysis was performed between a high expression of my testing gene and...
28 March 2022 2,894 2 View
We want to design antisense oligonucleotides for suppressing the gene of interest. The databases or related literatures found are either old or irrelevant. We are new in this field. Are there ASO...
23 March 2022 5,457 0 View
Hey everyone, I am inclined to conduct an experiment during which I wish to monitor the heart rate of participants and analyse it once the experiment is over. Ideally, the device used for this...
22 March 2022 3,316 6 View
I work with primary cells (explant culture) and while performing IF staining, I require several washing steps. As the culture is a tissue, it seems to get dislodged by the time I perform all the...
17 March 2022 5,156 3 View
I am facing some issue regarding the sample preparation of FFPE tissue for the MALDI mass spectrometry Imaging. As many protocols suggest I tried pulse boiling the slides in a citric acid...
17 March 2022 9,462 3 View
Hi Have anyone experience in purifying DNA from FFPE tissue from islets of Langerhans or is it not possible?
16 March 2022 4,252 2 View