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Hello everyone, Any references or insights from previous studies would be greatly appreciated. Thank you in advance!
24 July 2025 7,451 2 View
Which input modalities, pose-estimation approaches, gait features, and machine learning models best detect gait abnormalities in elderly individuals and accurately predict their future disease...
23 July 2025 3,380 1 View
Hi everyone, I’m working on mouse dorsal root ganglia (DRG) samples that were: Fixed in 4% paraformaldehyde (PFA) for 24 hours, Embedded in OCT compound, Stored at −80°C. I’m considering...
21 July 2025 7,577 2 View
إلى أي مدى يمكن لتطوير قطاع الخدمات أن يسهم في تحقيق التنويع الاقتصادي في الجزائر، وما هي العوائق البنيوية والسياساتية التي تقف أمام تفعيل كامل لإمكاناته؟
21 July 2025 2,922 3 View
Hello, I am Md Rofiul Islam Rofi. I am a recently graduated student. I am graduated in textile engineering .My research fields are environment, chemical, textile fiber, materials, factory waste...
20 July 2025 4,727 2 View
I've had excellent results staining perfused mouse brain tissue fixed with 4% PFA using Iba1. However, I’m encountering difficulties when working with fresh frozen tissue—no staining is...
19 July 2025 4,537 0 View
I have to find out the rainfall over a district from IMD gridded rainfall dataset for that i have taken arithmetic average of all points lying in that district. Is it a valid approach. Or I need...
18 July 2025 5,547 2 View
All around, we hear that IT cells are creating chaos and misleading people. What exactly are they, and how can we protect...
18 July 2025 6,738 0 View
Hi everyone, I would like some help on how to test to see if my primary antibody is still working. As this was at the beginning of my PhD, I wasn't very sure on how to store my antibodies, and I...
17 July 2025 9,662 3 View
In the context of tiered support framework (MTSS) implemented in primary schools (and Kita/preschool), Germany, we are exploring tools that are helpful for research children's behavior in the age...
14 July 2025 1,124 2 View
Has anyone worked with Ac4ManNAz? I’m wondering if it’s classified as an azide compound and whether it poses any explosion risks. Would really appreciate some insights!!
11 July 2025 8,566 1 View
I am working on epigenetic biomarkers of oral cancer (DNA methylation) using MSP. What should be the method to calculate sample size? My study is unmatched case control; control would be healthy...
10 July 2025 3,288 0 View
Dear colleagues, I’d like to discuss a technical question regarding stereotaxic injections in mice. During bilateral injections, I’ve observed occasional asymmetry in DV coordinates between left...
10 July 2025 2,205 4 View
It is requested to all of you kindly provide your valuable inputs with this regards. I will be very thankful to all of you.
09 July 2025 9,125 3 View
1. Is recombinant TGF-β1 protein widely utilized to establish in vitro and in vivo fibrosis models across different tissues (such as lung, liver, kidney, or skin)? 2. What are the standard...
08 July 2025 6,178 1 View
If a researcher purchases the same chemical from different vendors, there can be variation in solubility. The following factors can affect the solubility of the same chemical: solvent of...
05 July 2025 5,411 2 View
(Quantifying RNA Libraries for Ion Torrent Childhood Cancer Panels)
05 July 2025 9,491 1 View
Although several genes like C9orf72, TARDBP, FUS, and GRN have been associated with familial ALS and FTD cases, the majority of cases remain sporadic, and the underlying mechanisms are still...
04 July 2025 8,495 1 View
Seeking CCL3 inhibitors that effectively block the CCL3-CCR5/CCR1 axis in AD, preferably with evidence of crossing the BBB and modulating neuroinflammatory pathways (e.g., NF-κB/BACE1...
03 July 2025 3,711 1 View
I am doing a DNA extraction of blood of Mergus octosetaceus with phenol-chloroform, and when I put the TrisNH4Cl (5M) 1000ul, it makes the solution homogeneous, so even after I centrifuge the...
02 July 2025 3,419 2 View
Hi everyone, I’m planning to prepare an L-DOPA solution for cell culture. According to the data sheet from Sigma, the solution should be freshly made because L-DOPA oxidizes quickly when exposed...
01 July 2025 6,233 3 View
I'm currently estimating total protein content in cowpea seed samples using both CHNS elemental analysis (based on nitrogen content) and Lowry's colorimetric assay. in my case, the Lowry values...
30 June 2025 7,986 4 View
Good day everyone i want to ask, does anyone ever work in positional distribution of fattty aciid at sn-1 and sn2 position using lipase enzyme? If yes, how can you get it; please teach me the method
27 June 2025 4,075 2 View
Dear all, I am currently looking for software capable of assessing radiation dose in CT imaging and visualizing the spatial distribution of the dose within the scan. The software should be able,...
26 June 2025 7,089 6 View