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Questions related to Tissue
Why do we use non-fat or skim milk in blocking during ELISA and WESTERN BLOT, why it is only normal milk? what is the science behind "non-fat"?
22 July 2022 5,144 1 View
We have used Zymo's Direct Zol -96 RNA and Microprep column kits and have only obtained very low yields and purity.
21 July 2022 9,210 0 View
I would like to know if stem cells can be helpful in repairing a damaged organ of body and how
19 July 2022 715 1 View
I have a question which is crucial for my research, do we have any estimated proportion of how much sapiens lived in caves, rockshelters or open-air sites, if you know any available data about...
19 July 2022 4,101 4 View
Hello everyone, I would like to try hypoxia/reoxygenation in vitro using neonatal mice cardiomyocytes with a hypoxia chamber. I read many models in the litterature but I don't know which one is...
18 July 2022 8,518 1 View
I have been working with 40uM mouse brain sections mounted on triple coated chrome alum gelatin coverglass for multiplex imaging. By Round 2 I see these fluorescent specs/precipitate/debris all...
18 July 2022 9,194 0 View
Blood cancer is a very dangerous disease. Blood can circulate to all parts of the body. Blood cells are generated in the bone marrow. So I decided that I work on both blood and bone cancer and...
17 July 2022 8,777 3 View
I am currently working on a project that aims to measure the protein activity of purified recombinant eNOS. If our protein is active we can continue with binding assays. We attempted to use a...
13 July 2022 1,504 0 View
Does anyone have experience in NCOA4 WB?
10 July 2022 6,787 0 View
I have whole genome of the Monkeypox virus. Is there any webserver for its genome analysis
09 July 2022 6,143 0 View
Which NGS technology (if viable) would be most useful in confirming presence/absence of virus contamination in cell banks used for drug production? I imagine it would include WGS of host cell...
06 July 2022 9,011 0 View
The question is meant to compare my own downward divisions with coming opinions. My divions are: 1) Increased CSF pressure as main target of treatment is to normalise it. 2) Residual unchangeable...
04 July 2022 5,983 0 View
My question is about standard DVB-RCS2 in ETSI EN 301 545-2 V1.3.1 (2020-07) (https://www.etsi.org/deliver/etsi_en/301500_301599/30154502/01.03.01_60/en_30154502v010301p.pdf) I would like to...
04 July 2022 5,999 0 View
How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?
04 July 2022 5,991 3 View
Hello. I have to isolate PBMC from CPT tubes with sodium heparin and the centrifugation is without break? Could you help me with this? My centrifuge is: Brai4, Jouan. Thank you.
04 July 2022 7,482 0 View
I have mitochondrial pellet with me kept in homogenization buffer. Now i need to perform BCA. Kindly confirm how much quantity of RIPA Lysis buffer and protease inhibitor is to be added to that...
30 June 2022 8,286 0 View
Dear researchers, Since WQI models are not generic and there are more than 35 ones, what is the suitable model (WQI) to assess spring water quality?
29 June 2022 2,644 3 View
I weigh 100 mg of tissue. I don't cut open the tissue and wash with room temp PBS. I use 300ul ripa while homogenisation and add 200ul after that.
24 June 2022 406 1 View
I want to get the OR with 95%Cl and a forest plot.
22 June 2022 8,343 0 View
I am culturing MSCs on 3D-printed hydroxyapatite scaffolds. We need to detach cells from the 3D to analyze/quantify overall DNA content using Quant-iT PicoGreen dsDNA Reagents and Kit. However,...
21 June 2022 5,701 2 View
Can the RNA be run on DNA HS ChiP Bioanalyzer just after first strand synthesis? Or is it required to do the second strand synthesis as well (complete cDNA) before running it on the bioanalyzer?
14 June 2022 6,433 0 View
Good day scholars. Please I need to determine the dopamine level in the brains 🧠 of experimental rats 🐀. I don't want to use ELISA; the alternative method I got requires spectroflurimeter which I...
11 June 2022 6,728 0 View
I have estimated proline in sugar beet leaves and have observed the absorbance at 520 nm. Now I want to make a standard curve for further estimation and calculation of proline. Can anyone help me...
02 June 2022 8,461 7 View
Please treatment would you recommend for my mortar and pestle as well as the magnetic stirrer that got stained (with black) after using them for magnetic nanoparticles synthesis? Thank you
26 May 2022 3,484 6 View