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Questions related to Tissue
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23 January 2024 1,132 2 View
For under canopy planting, what is the standard minimum requirement of light quantity reaching through created gaps for the early growth of understory species; I do understand that the light...
23 January 2024 679 0 View
Succer fish live on detritus food.
23 January 2024 3,026 1 View
Hi to all Hope you are doing well. I am amplifying Kan cassette in PkD4 plasmid with 40bp extensions in primers to knockout gene in MG1655 E.COLI by lambda red mutagenesis . the expected product...
22 January 2024 9,061 1 View
I have used STAT60 to isolate RNA successfully. I am wanting to collect RNA and protein from tissues, and according to the manufacturer's information I could allegedly use STAT60 to collect both...
19 January 2024 2,631 1 View
I am conducting a systematic review and meta-analysis of many studies with overlapping populations. The Review Manager 5.4 software does not require a sample size to perform a meta-analysis of...
19 January 2024 4,918 1 View
I am trying to establish a PK/LDH system to detect ATP turnover. We are having difficulty observing any measurable change in NADH fluorescence (we are not using the absorbance approach), and...
17 January 2024 2,663 1 View
I am using SOX1 and Nestin as primary antibodies (1:200 and 1:500 for each) and Anti Goat Alex Fluor-594 and Anti Mouse Alexa555 as secondary antibodies (1:1000) and still not getting any results....
16 January 2024 9,632 2 View
Why would PD-L1 degradation result in limited proliferation of MC-38 cells?
16 January 2024 7,790 2 View
I need help with selection ADA confirmatory assay format. Does anyone have the experience of selection confirmatory assay among ELISA, SPR?
15 January 2024 8,904 0 View
How do chemical fertilizers affect soil health?
15 January 2024 6,472 3 View
How can learning strategies and educational curricula be adapted to better align with the dynamic needs of the contemporary job market, and is there a feasible model for integrating real-world job...
11 January 2024 5,105 8 View
Oligo length is 19bp
11 January 2024 4,286 1 View
How would you define effective teaching in your context, taking into consideration the expectations of both students and the institution?
06 January 2024 5,722 4 View
Hello, I would like to know the concentration of collagenase that dissolved in P2 perfusion (Hepatocyte organoid) This is because the liver does not break down easily and there are not many live...
05 January 2024 7,816 2 View
I am growing Arabidopsis (Col-0) in both +P and -P medium (JOHNSON'S medium) the plants which are growing in -P showing the anthocyanin accumulation in their leaves when it compared to plants...
02 January 2024 10,004 3 View
Dear RG Member, I hope this message finds you well. I have a set of 50 natural product ligands and plan to conduct molecular docking studies for a skin cancer research project. Could you kindly...
02 January 2024 1,863 2 View
My current problem is that my cells die when I grow them. It is an MC38 cell. The medium I use is RPMI modified, without calcium nitrate, with 2.05 mm l-glutamine. I add 10% FBS and 1%...
01 January 2024 5,158 3 View
I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?
31 December 2023 7,655 0 View
In my study, all films were grown on the stibnite (polycrystalline) structure of Sb2S3. When I changed a parameter of a group of films, I observed that XRD peak intensities increased, while Raman...
30 December 2023 5,411 5 View
Fish feeding
28 December 2023 1,875 3 View
I am first time doing bacteria experiment without any prior practice. I am trying to make bifidobacterium biofilm. According to references i am incubating them in different concentration of bile...
24 December 2023 7,568 5 View
Hey all A few of my lab-mates store the protein after quantification (I used bradford assay) in cell lysis buffer containing protease inhibitor in -80. While others add loading dye and denature...
24 December 2023 9,815 4 View
Hello, Kindly what are the recommended methods or techniques for removing XRD impurities and how can one ensure accurate and reliable results ? Thanks.
21 December 2023 4,667 18 View