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Questions related to Tissue
I find myself in a predicament that I'm not sure how to resolve: I have managed to isolate a particular multi-cellular structure from postmortem human brain tissue with the intention of isolating...
23 December 2021 5,650 1 View
Kindly suggest me that ..... Which test will provide an exact evidence about mitochondria (morphology - tissue level & quantification in blood using plasma) estimation of Wistar rats If you...
18 December 2021 8,076 0 View
How to dissolve sodium citrate in ethylene glycol in a short time?
15 December 2021 5,119 1 View
I have performed human whole-genome sequencing using NovaSeq6000 and analyzed the data using Basespace. Now I got some results for breast cancer 28 genes list which has been assembled in an excel...
15 December 2021 2,529 1 View
In case of Toxoplasma gondii infection tissue cysts produced as a defense mechanism against the host immune response .
11 December 2021 3,155 4 View
HI All, My team is looking at the changes in distribution of the OTR in a range of male reproductive tissue. Currently getting non-reproducible results for RAT testis and hippocampus positive...
10 December 2021 4,723 2 View
Azoospermia, severe oligozoospermia, moderate oligozoospermia, or mild oligozoospermia are all symptoms of Y chromosome infertility. Males with Y chromosome infertility usually do not exhibit any...
10 December 2021 6,125 2 View
Hello, some papers report the density of bacteria in the whole colon. For instance, both O’Hara et al., EMBO reports 2006;7(7):688 and Sender et al., Plos Biol 2016;14(8):e1002533 give 1011...
07 December 2021 3,712 2 View
Hi, Just wonder if a rejected paper can be reposited in BioRxiv? As I see in Wiki saying: "In general, most publishers that permit preprints require that: the authors disclose the existence of...
06 December 2021 8,087 5 View
A cellulase enzyme was purified and volumes in microliters were drawn to breakdown CMC as substrate in enzyme characterization studies. How do I calculate the enzyme concentrations in microliters...
06 December 2021 4,794 3 View
Hi, the important step to analyze transcriptome data is the functional enrichment, of which GO and KEGG is the most popular. My question is how to assign the GO or KEGG numbers to my bacterial...
05 December 2021 5,584 2 View
Hi, So the whole blood samples are in RNA later solution and kept at -20 (500 ul blood+ 1000 ul RNA later). Please suggest protocol to discard the RNA later and process for RNA isolation. These...
04 December 2021 1,380 2 View
I extracted the sample from whale tissue with deterioration stage 4. I need to amplify the nuclear trait of the alpha-lactalbumin but also by increasing the amount of template the resulting band...
03 December 2021 7,093 3 View
My group and I have some samples of frozen mouse brains that are fixed in paraformaldehyde. However, tissue quality does not allow us to perform the immunofluorescence protocol. So, so that we...
03 December 2021 5,937 2 View
Hi there, I am trying to do two-channel light sheet microscopy for a whole mouse oviduct, but the autofluorescence from the vessels is masking my true target. I am currently using PFA for fixation...
02 December 2021 4,730 1 View
Hello I'm studying characteristics of students' language when they are engaged in covariational reasoning. Meanwhile, I followed an inductive approach to characterize categories. However, I'm also...
02 December 2021 4,529 4 View
- why was there constant washing with BSA many times? - why did we use these materials for ICC? What are the alternatives? - why we have seen the actin filaments?
29 November 2021 1,336 3 View
I intend to collect data on goldfish fecundity which I reared semi intensively. Which will be the best way to collect data on fish fecundity?
26 November 2021 2,510 3 View
I intend to use sucrose gradient, but don't know what speed to use and how long. Any suggestions would be appreciated
26 November 2021 2,593 0 View
These creatures are commonly present in a pollen slide from the Early Pleistocene in Holland, which flora is dominated by Picea and Pinus with some Tsuga.
26 November 2021 6,651 10 View
I use 0.1% Sudan Black B in my brain immunofluorescence staining, even though it reduces autofluorescence of background but also gives low non-specific staining. It looks like neuronal marker...
23 November 2021 6,067 6 View
I'm looking for a company where I can buy pestles or potter sticks to crush samples in microcentrifuge tubes/pcr tubes. I can only fing potter sticks for 1.5 mL tubes.
23 November 2021 9,988 4 View
Hello everyone! I recently started working with 4T07 and 4T1 cells. To do an IF experiment, I've seeded the cells on the coverslips. The 4T07 cells look completely fine and healthy, however, the...
23 November 2021 5,024 0 View
In Radiotherapy, in monaco treatment planning system the range of elecron denisty is from 0 to 2.4....can I extend this range?
22 November 2021 2,459 0 View