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Questions related to Tissue
Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...
21 December 2023 7,903 0 View
I measured the Thickness of the thin film using ellipsometry. But, now i want to study the swelling of thin film using 500 ul liquidCell alpha ellipsometry. Looking for some reference papers and...
20 December 2023 9,214 5 View
Hi everybody, I have a tri-layer vascular Graft ( one layer is TIPS, one is electrospinning and the other is ECM) I should Insert each end of my VG in a luer inside my bioreactor but I would...
19 December 2023 6,236 1 View
What are the ethical implications of cloud computing, especially in terms of data privacy and responsible AI development?
19 December 2023 1,142 9 View
Most of the commercial antibodies available seem to target the T18 domain (aa 225-339) of the adenylate cyclase, or they do not specify which domain. I am in search of an antibody specific to the...
19 December 2023 180 0 View
Dear all, I am currently working on a protein simulation with a heme group in the protein. I am wondering whether anyone can tell me how I can set it up in GROMCAS with charmm forcefile, since...
18 December 2023 4,422 2 View
MenBvacen
18 December 2023 9,986 2 View
I have 10-30 micro thickness FFPE slices. I want to extract metabolites for LC-MS analysis.
18 December 2023 6,096 3 View
What is recent research on fentanyl and its derivatives determination by differential Raman spectroscopy?
16 December 2023 9,934 0 View
I am currently working on the T24 bladder cancer line and I am co-culturing for 72h with immunosuppressive myeloid cells generated in vitro. After 72h of co-culture, I want to isolate T24 cells by...
16 December 2023 2,926 0 View
I am doing immunofluorescence of mouse brain cryosections. I collect 25 um sections onto 1% gelatin coated coverslips, let them dry ~30 min, then peel off the OCT, rehdyrate the sections with 0.1...
15 December 2023 4,077 0 View
Hello everyone, I would like to use platelets in a co-culture with other cells. I was wondering which are the conditions to keep alive the platelets: -since I am going to buy my platelets, I was...
15 December 2023 2,372 10 View
basically, we are conducting a electrocoagulation experiment and will be using this process to reduce nitrate to a certain level. However, we are struggling on what reagents to use since the...
13 December 2023 3,726 3 View
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13 December 2023 8,455 3 View
Hello Research Gate Community, Wondering if anyone has had issues migrating from the RNAscope Multiplex Fluorescent Assay V1 to the V2 version for mouse brain tissue? We had the V1 kit working...
12 December 2023 3,216 0 View
12 December 2023 6,587 5 View
Hi! I want to deproteinize a sample and then apply the resulting deproteinized product to cells in culture (PBMCs). Which method can I use for this? I thought about TCA, but I am not sure whether,...
11 December 2023 3,587 4 View
How can i know if it microporous, mesoporous or macroporous or moixture by mere looking is there a way to analyse before further using physisorption?
11 December 2023 730 2 View
The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...
11 December 2023 5,704 2 View
explain using biological basis and the nervous system
10 December 2023 5,341 0 View
10 December 2023 3,974 7 View
Hi everyone, I am trying to peel out the intestine layers using the traditional way, but it's hard and time-consuming. And looking for an enzyme or chemical agent that might help with breaking...
09 December 2023 2,277 0 View
This parasitoid was identified from cocoons adhering externally on caterpillars of Parotis marginata (Hampson, 1893), Crambidae. I could guess that this species belongs to the subfamily...
09 December 2023 9,238 0 View
Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the...
08 December 2023 5,004 3 View