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Questions related to Tissue
I've been generating and culturing multiple different human iPSCs lines on mito-MEF feeders using standard hiPSC media composed of Advanced DMEM/F12 (Gibco) with 20% KSR, pen/strep, glutamax,...
19 November 2021 6,103 0 View
I have two unique teleost species, Saffron Cod and Broad Whitefish. I am analyzing the HSP70 concentrations in the cranial, hepatic, and muscle tissues. The Broad Whitefish samples all worked...
18 November 2021 3,073 2 View
Hi everyone, I am cutting fish larvae after their embedding in Technovit 7100. I usually use methylene blue/azure II/basic fuchsin in the staining phase. However, I noticed that for some tissue...
18 November 2021 1,511 3 View
Hi all, I recently started using a new Olympus system which saves multiple image files as a .ets file with an associated with a 'master' .vsi file. If I understood correctly, the vsi file...
18 November 2021 4,381 1 View
We do not have GC/MS available in our Lab, therefore I need to send Tissue samples to the company for metabolomic analysis. The out source Lab has to perform derivatization and extraction and...
15 November 2021 5,774 3 View
Are there any specific classes in geant4 for nano-materials? Or anyone could share an example of detectorconstruction.cc for simulating nanoparticles induced detectors?
15 November 2021 8,974 3 View
ICGC/TCGA takes a sample of the cancerous tissue and a sample of the normal tissue for each patient. I would like to gather the Renal cancer patients of ICGC for example, and get the mean...
14 November 2021 205 1 View
Dear All, Can microsomes be obtained from liver tissue without ultracentrifugation for in-vitro drug metabolism? Thank you.
11 November 2021 7,754 3 View
I am analysing LC-MS data. I have combined all NEG and POS mode peak intensities in a single file retaining all metabolites with higher peak intensities (appearing in either modes). Now, when I go...
10 November 2021 4,690 9 View
I’m working on spinal cord mice tissue and I want to satin motor neurons with luxol blue and cresyl violet, so does anyone have a working protocol?
08 November 2021 1,883 2 View
Dear all, I have followed the below protocol to assess the MDA (malondialdehyde) in the mouse optic nerve. As I know the reaction between MDA and thiobarbituric acid (TBA) should yield pink color...
06 November 2021 320 2 View
I collected root samples from different tree species at different sites in different seasons. Every time I processed the samples until the step before DNA extraction and stored them. Samples have...
05 November 2021 5,055 3 View
Dear all, I would like to quantify protein nitrosylation but most ELISA kits are very expensive. I have already quantified the amount of nitrites in cell cultures using the Griess Reaction. Is...
04 November 2021 1,177 1 View
I'm working with primary human synovial fluid cells (neutrophils, monocytes, etc) and PBMCs. The PBMCs do great with my typical freezing and staining protocol, but the synovial fluid cells are far...
03 November 2021 6,418 0 View
For example how can I search for expression changes in x tissue induced by armodafinil consumption? I do not know how to navigate the site
02 November 2021 4,835 2 View
Can we use deionized water instead of mounting medium for confocal microscopy to preventing photobleaching? Since all the parts of the tissue does not mix well with thick mounting medium and...
01 November 2021 7,066 1 View
I am looking at staining mouse brain tissue for three different proteins (TPH-1, FFAR 2, and IL-6). The tissue is fixed (24 hours in paraformaldehyde twice, and then 2 rounds of 24 hours in...
30 October 2021 9,431 3 View
I want to perform a in situ hybridization to see where my gene of interest being expressed but I haven't performed in situ hybridization for Drosophila brain. Can anyone share his/her protocol or...
28 October 2021 8,158 0 View
Hi. I am trying to detect cell death using TUNEL method in sea urchin larvae. Although I was able to stain ectoderm, I have not stain mesoderm and endoderm. I want to know weather cell death...
28 October 2021 7,759 0 View
I would like to perform in-situ hybridization and use ready-to use probes. Any recommendation ?
27 October 2021 1,749 1 View
It's an idea to create a rapid test kit, with some data backing up that this so the test can have a certain degree of acceptance for the honey quality, (in terms of adulteration). A majorly...
27 October 2021 100 5 View
Hi everyone, Does anyone have experience growing tissue slice 3D models of any cancer in organoid mediums and/or if they would be useful to increase the lifespan/stability of tissue slices? I am...
26 October 2021 5,967 1 View
Any articles or protocols related to the preparation of tissue lysates from freshly resected tumor samples for Western Blot would be appreciated
26 October 2021 2,178 3 View
Hello! For our current research project, we want to analyse (as simple as possible) the dendritic spine density of our mouse brains. Apart from the Golgi staining, which methodology could be...
25 October 2021 4,382 5 View