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Dear Community Members, I'm reaching out to you, especially to those specializing in the medical field, for information regarding clinical trials on patients who have suffered from the rupture of...
05 June 2024 1,444 0 View
Hi everyone, I need help and yuoue experience!!! What kind of cell culture contamination is it? Video attached: The cells are lung cancer cells that are thawed from -80°C fridge and we known that...
05 June 2024 9,597 2 View
To the best of my knowledge, a systematic review aims to collect and summarize all the published & unpublished literature revolving around a certain topic/sub-topic. Sometimes, I encounter...
04 June 2024 6,368 3 View
《Plant Nutrition Diagnostics: Potato》
04 June 2024 1,645 2 View
In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...
01 June 2024 5,187 0 View
Hi everyone! Lately I've been running into the problem that in some lanes in my SDS-page gel, the sample is not running correctly (see picture) . In these cases the rest of the gel, based on...
01 June 2024 6,854 3 View
I tried western blot targeting TTF-1 (Thyroid transcription factor-1) also known as Nkx2.1. I tested two primary antibodies, one is Invitrogen, Nkx2.1 Monoclonal Antibody (8G7G3/1) and the other...
30 May 2024 3,499 3 View
I am using a rectangle Ni foam electrode area: (0.5 * 1 = 0.5 cm2) for OER and applying material to the area (0.5 * 0.5 = 0.25 cm2) and depositing the same area in the solution. Now, do I need to...
30 May 2024 1,429 5 View
I am trying to get a CV of Hydrogen Peroxide on Prussian Blue which I electrochemically deposited on screen printed carbon electrode. I am using hydrogen peroxide as the signature molecule to...
29 May 2024 1,519 2 View
I am using mouse bone marrow derived MSCs (passgae 3-6) for my in vitro cytokine priming experiment. My basal media is 2% FBS-DMEM. I am using 10 ng/ml TNF-a, or IFN-g or both for 24 hours. I see...
28 May 2024 8,914 0 View
I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after...
28 May 2024 4,756 2 View
I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...
28 May 2024 1,576 1 View
Dear Experts, I am currently conducting a Molecular Dynamics (MD) simulation using the NAMD package to observe the formation of a two-phase system between glucose, acetonitrile, and water. To...
27 May 2024 5,690 2 View
Hi, I'm doing research n-type neuromorphic transistor device. According to theory and other research, forming EDL (electro double layer) can cause anti-clockwise transfer curve. However, as you...
27 May 2024 5,384 1 View
Hi, For compensation, we can use single-stained beads in flow cytometry. For gating control or FMO: can we just mix single-stained beads to get FMO?
26 May 2024 6,809 1 View
I was doing my research about brucellosis, the study aimed to relate the demographic variables of target animals( e.g. age, sex, parity, animal vaccination history, etc.) to the occurrence of the...
26 May 2024 6,894 0 View
I am trained in Physics, but I spent my PhD doing biomedical research (wet lab). Now, I want to continue my career in biomedical research without losing my background as a physicist. So, what is...
26 May 2024 6,563 3 View
A new compound that is available for purchase to inhibit the particular target which have no literature regarding its physical properties, how will I calculated its melting or boling point for...
25 May 2024 2,891 4 View
Hello: I'm working on a database that includes catches from fishers who are stringing nets together with different mesh sizes. To reduce inconvenience for the fishers, the fish data were pooled....
23 May 2024 2,896 0 View
The first questionnaire contains responses of parents as the independent variable. The second contain responses of middle age children as the independent variable. How do I go about inputting it...
23 May 2024 5,715 5 View
Commercialization of space activities and private sector investment requires the creation of a new legal system that creates the necessary incentives . Are the basic principles of space capable of...
21 May 2024 4,905 1 View
Hello everyone, I'm administering subanesthetic doses of ketamine to mice (10 mg/kg; i.p.), and I noticed that their motor coordination becomes compromised for up to an hour following injection....
21 May 2024 790 4 View
Specially for experimets conducted in multiple environments
20 May 2024 2,928 0 View
Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?
19 May 2024 6,841 2 View