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Questions related to Tissue
Hello everyone. I'm doing an evaluation of microplastics in several coastal species tissue samples, and what I want to know is what is the volume ratio of solvent:tissue to be used in the...
06 October 2022 5,444 5 View
I transferred KB cells stored in -80 into a T-75 flask by adding 10% FBS media without centrifugation. I plan to change the media after proper adhesion of the cells. I don't know how many days it...
05 October 2022 983 3 View
I can't access mirtarbase (mirtarbase.cuhk.edu.cn or http://mirtarbase.mbc.nctu.edu.tw/) because its says that "the requested URL was not found on this server". I’m wondering if it's down...
29 September 2022 1,520 1 View
I wanted to test the effect of enzyme on drug dispersion in tumor tissue, and administrated fluorescent microparticle (1-10uM) under mouse xenograft tumors by intra-tumoral injection. - tumor...
27 September 2022 5,727 0 View
I am currently using NH4OH to elute a protein from a bead-antibody complex in order to analyze it by mass spectrometry. Compared to previous assays we obtained fragmentation of the protein, so I...
23 September 2022 8,568 2 View
I have been growing E. coli and P. aeruginosa strains in my biofilm formation assays. However recently, after washing the crystal violet off with water, there appears not to be any rings of...
11 September 2022 9,715 0 View
What should be the optimum pH of the hydroponic medium treated with Arsenic and Silicic acid for better biomass of paddy seedlings? Thanx in advance.
09 September 2022 3,830 0 View
Hello there, We have had the Thermo Scientific™ HM 355S Automatic microtome for a while and nobody in our lab is using it. I was wondering whether you could help me with how to remove the...
05 September 2022 410 3 View
I am currently working on the femur bone. I need CT scan data for the femur using mimics. Where can I download Dicom images of the femur with the patient's age, weight, and gender...
04 September 2022 3,081 4 View
Sample quantities would be less than 100grams and for scientific R&D purposes only, no commercial value. What kind of documentation or forms should I prepare?
01 September 2022 5,487 1 View
I am working on electrocatalytic nitrate reduction from a water solution. I want to measure the concentration of nitrate, nitrite and ammonia available in water solution at different times using...
01 September 2022 4,521 3 View
I have PBMC and serum to transport to a lab in another country yet I can't get dry ice on my flight day
31 August 2022 5,099 2 View
Hello! I'm trying to differentiate MG-63 cells into osteoblasts and have no idea..:( I found that this cell line is osteosarcoma cells and I have no idea how cancer cells can differentiate into...
29 August 2022 5,145 0 View
Has anyone got information about plastic tubing and the impact on stem cell growth? I am needing to change the tubing for the CO2 gas tank to the incubator and I want to avoid any potential...
26 August 2022 2,193 1 View
I am interested in some cytokines, such as IL12, IFN-gamma, TNF-alpha and IL2. For my latest research study, I would like to purchase these proteins but in a fluorescently-labelled form. Does...
20 August 2022 4,066 2 View
I am working on thin film semiconductors. From XRD results what I observed is an increase in the crystallite size with an increasing temperature. Can I say an increase in the crystallite size is...
16 August 2022 4,864 4 View
I want to screen some tomato germplasm in the field for drought stress, with 50 % field capacity how can I measure FC?
12 August 2022 3,531 3 View
Interestingly A group of whales appeared near the shore to feed for a long time about 15 days at Hon Trau, De Gi, Phu Cat District, Binh Dinh province. Ask the experts to help identify this...
12 August 2022 5,680 3 View
Hello, I've been trying to plot the data that I get from the QuimP plugin on ImageJ. I just need to plot the coordinates, X and Y, and follow them during a period of time. At the end I need a plot...
10 August 2022 9,554 0 View
-does Triton X-100 also permeabilize nuclear membranes? -what is the ideal concentration of Triton X-100 to use? -do low temperatures slow down or prevent cell lysis (by Triton)?
10 August 2022 1,891 0 View
WI-38 cell yield from T75 flask
09 August 2022 5,157 0 View
Hypertensive crisis is an umbrella term for hypertensive urgency and hypertensive emergency. These two conditions occur when blood pressure becomes very high, possibly causing organ...
28 July 2022 2,853 1 View
I want to Immunostain retina vasculature, so I'm looking for a protocol to embed the retina in wax, do I need to embed and section the whole eye or do I have to remove the cornea and the lens and...
27 July 2022 5,031 2 View
I want to conduct a shade tolerance - drought tolerance experiment for tree seedlings from seasonally dry tropical forests of northern Western Ghats
24 July 2022 3,294 4 View