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Hi do anyone know of a standard vector that can express GST without any fusion protein? As I understand it is that for many standard vectors that uses GST as fusion protein doesn't express GST...
25 September 2025 4,041 1 View
How to make thin films from a 3 inch diameter Li target (99.9% purity) in a magnetron sputtering device. Li oxidizes quickly in air, so I'm hesitant to use it. I'm currently storing it in oil....
24 September 2025 1,718 2 View
Poverty eradication in sub-Saharan Africa is most effective through a holistic approach that integrates both government-led and private sector-driven strategies, recognizing that neither can...
24 September 2025 5,412 1 View
Both companies face lawsuits over inadequate protections against child sexual exploitation on their platforms.
21 September 2025 4,659 8 View
Hi everyone. Recently i cloned my gene of interest in pLENTI-GFP vector and after the transformation i got the colonies positive when done with GOI specific set of primers and at the desired band...
21 September 2025 2,575 3 View
How does INRM contribute to climate resilience, livelihood security, and poverty reduction in semi-arid regions like Bundelkhand?
20 September 2025 6,863 0 View
Stained KYSE with CFSE and M1 macrophage with ER Tracker Red and incubated for 2 days. No fluorescence was detected under the fluorescence microscope. Any suggestions?
19 September 2025 4,857 1 View
Hello, I have histological images of adipose tissue and would like to quantify adipocyte size using ImageJ. The images do not include a scale bar, and the metadata is not accessible. The only...
18 September 2025 2,755 2 View
Lately, I have been having more of a difficult time lifting my stem cells during passages. I am currently using rhlaminin-521 to coat my plates and versene to lift the cells. Previously, I was not...
18 September 2025 984 0 View
"I am researching long-range smoke detection systems for industrial environments such as warehouses, factories, and high-ceiling facilities. Which technologies or products currently provide the...
17 September 2025 6,022 0 View
Hello , I am very interested in cardiovascular surgery and i am looking for doctor who is interesting in doing a research in cardiovascular grafting after trauma also severe illnesses
17 September 2025 6,923 0 View
In test–retest reliability studies, many older studies (around 1998–2008) only reported a “correlation coefficient” without specifying whether it was ICC or Pearson. From today’s methodological...
16 September 2025 5,991 0 View
VOICES OF REFORM in Math Education Educational Research to Inform and Reform Voices of Reform is a double-blind, peer reviewed journal aimed at bridging the gap between scholars and practitioners...
15 September 2025 4,593 1 View
The calibration curve was performed incorrectly, and certain reagents are not available to perform it again.
15 September 2025 4,240 2 View
Hi all, I’m currently working in a neurotoxicology group and performing qPCR experiments using BV2 (mouse microglia) and HMC3 (human microglia) cell lines. While using the same cDNA input amount...
10 September 2025 4,861 1 View
Hello I want to check the presence of a gene on semen with FISH. I don't have a large probe, so I need to design an oligonucleotide probe. I heard that due to the weak signal, I should do first in...
08 September 2025 5,217 2 View
I want to update my professional email address
08 September 2025 1,927 2 View
My PhD research is a comparative study of the economic development trajectories of five countries: Ghana, Algeria, Angola, Côte d’Ivoire, and the DRC from the 1950s to the 1990s. I am exploring...
05 September 2025 9,441 0 View
Both plasmid at different position is it due to repitation or already found 2 plasmid?
02 September 2025 6,680 2 View
What is InTech Open Science? A predatory or a ligitimate publisher?
31 August 2025 6,846 1 View
I am a beginner in protein purification and currently learning to use the AKTAprime plus system for His-tagged protein purification. I would like some guidance on practical...
29 August 2025 5,819 2 View
A landmark of scientific revolution was to unify the aristotelian 2-realm concept:otgr laws apply for celestial objects and other for earthly..Everything was forced motion, not just earthly...
29 August 2025 8,395 0 View
I have run the program for Counterpoise correction for BSSE, but the program got terminated, saying "The combination of multiplicity 1 and 205 electrons is impossible". How do I solve this issue?
29 August 2025 1,534 1 View
Global evidence has documented the isolation of vanA-positive Staphylococcus aureus (VRSA). However, during my recent search in the NCBI GeneBank database, I was unable to find a complete vanA...
27 August 2025 4,508 1 View