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Questions related to Tissue
What the benefits of Negative Poisson's ratio materials and structures applications in Tissue engineering?
15 February 2022 4,863 3 View
My project topic is hyperthermic procedure to treat cancer. Hyperthermia involves heating the cancerous tissue at a temperature above 42'C and the heating should be localized. I know the theory...
15 February 2022 9,090 3 View
Hello! :) I am looking for a protocol for lyophilization/freeze-drying (primary drying only) of organs/tissues to obtain at least 1 g of drying sample at the end of the process, temperature and...
15 February 2022 8,373 8 View
I have to check the radiotherapy plan and dosimetry distribution for a CT image that I have generated and need to compare it with other plan. Is there any free software to compute the radiotherapy...
15 February 2022 6,993 1 View
Paragonimiasis is a parasitic disease that can be transmitted through the consumption of infected freshwater crabs by mammals including humans. Infective larval forms of the parasite has been...
14 February 2022 2,908 6 View
Hi community, we isolate decellularized ECM from various organs and produce hydrogels of these. I am looking for protocols to label ECM or ECM hydrogels with fluorophores, biotin, digoxigenin or...
14 February 2022 8,671 2 View
I am trying to digest adult mouse brain tissue for cell isolation, and I am having a hard time getting past the enzymatic digestion step. Previously I used the Dissociation Kits from Miltenyi...
11 February 2022 3,073 3 View
What's the step of proteinase antigen retrieval in immunochemistry? Which kind of tissue does it apply for? Thank you very much!
08 February 2022 2,380 2 View
I labeled E2-BSA conjugates in the past. Unfortunately, the antibodies seem to have non-specific binding to BSA. It would be great if any of you have dealt with such problems. Thank you, Parvez
08 February 2022 5,192 2 View
Hello, I would like to ask if anyone has experience to treat cells with human plasma? Is it possible to use sodium citrate or maybe heparin tubes? Are there some pitfalls? I know that EDTA is not...
08 February 2022 9,386 0 View
Suppose in Brine shrimp lethality tes of phytochemicals, no death of shrimps observed at all tested concentrations. What would be the LC50 of the sample?
07 February 2022 3,896 4 View
Other than doing it by hand, can you please recommend a precise way to cut (longitudinally and horizontally) plant root tissue around a bending zone. As in the image, I want to separate 6...
05 February 2022 627 3 View
Hello kindly I would like to know how can I do freeze -dry to evaporate my sample instead of using TurboVap ( Nitrogen flow and water bath) as my sample contain water ? Best
05 February 2022 464 2 View
Hi! I have a question regarding the preparation of dextran-FITC solutions. As of now I have been preparing fresh my dextran-FITC solution to perform my experiments of dextran transport. However,...
04 February 2022 5,267 0 View
Good day, I would like to perform miRNA profiling from serum. Have anyone used the MGI sequencing platforms such as DNBSEQ-G50 o DNBSEQ-G400 de MGI? Is it comparable to Illumina in terms of...
03 February 2022 8,126 0 View
Dear all, We need to isolate cancer associated fibroblast from colorectal tissue and distant normal colon tissue. For this propose, we obtained specimen by surgery from a CRC patients. Our method...
02 February 2022 2,674 2 View
Hi Everyone, We need to attach metal beads to contact lenses for a project, and I could not find any adhesive that works with contact lenses (preferably biocompatible) . I know that they are...
02 February 2022 1,883 2 View
I am currently doing ex vivo work using ectocervix tissue. My tissue has a lot of cells migrating out but none of the cells are attaching on the plate. The cells seem to just be sliding on the...
01 February 2022 1,069 4 View
Hi! I have an issue during tumor dissosciation During the breast tumor dissociation (human, I use Miltenyi Kit for dossociation) I have poblems with cell counting. Automated cell couter...
01 February 2022 1,244 1 View
Hello. I am working with a membrane bound protein, and I've discovered, that after I dissolve and homogenize the tissue in RIPA buffer following by spin down, my protein of interest ends up in the...
31 January 2022 9,237 2 View
Which is a good method (model/system/individual stand) for individual feeding of dogs kept in group (8 to 10 animals in pen)?
30 January 2022 9,737 1 View
I am using Himedia DMEM with 10% FBS for the Aedes albopictus C6/36 cell line. They are getting lysed after few days of revival. I have tried everything like using fresh media and its components,...
28 January 2022 4,537 4 View
Hi, I am trying to load the apo-transferrin with iron but the procedure does not work. If successful, I am expecting to see the internalization of transferrin. I am following the protocol...
25 January 2022 1,673 0 View
With regards to the protocol by 10X Genomics:...
24 January 2022 8,299 5 View