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Hello, My collagues and I are trying to stain paraffin-embedded slices (mouse brain). After deparaffinization, hydration and dehydration ( as explained on...
20 January 2022 9,034 3 View
May I have any help about this? I can see some hyaline areas but what is that cell infiltration?
20 January 2022 6,927 2 View
I'm going to prepare a muscle frozen tissue sample ChIP with pan-acetylated H3 histone antibody. What inhibitors do I need to prevent spontaneous deacetylation of H3 and its degragation? What...
19 January 2022 7,187 2 View
Is there any database to get the chromosomal location or rs numbers of HLA alleles?
19 January 2022 4,106 0 View
I have tissue treated with fluorescently labeled EVs. What is the best way to process the tissue for sectioning that can retain the fluorescence? I have tried cryostat sectioning. But the tissue...
18 January 2022 3,988 1 View
Is it possible whole organ regeneration from decellularized organ in vitro?
18 January 2022 1,591 6 View
I am currently using the explant method to grow out primary cells from an ectocervical explant. The tissue was transported in media after surgery and immediately processed by cutting the tissue...
17 January 2022 5,953 1 View
Investigation of apoptosis in liver tissue
15 January 2022 3,110 2 View
Dear all, Recently I met a problem. We screened out a gene A which performs like a tumor suppressor gene. It negatively correlates with clinical patients' survival. And it affectes tumor cell...
12 January 2022 6,821 14 View
I have been using Biorad RNA synthesis kits which gives better RNA yield and quality from most of the tissue of breams, silver pomfret, seabass.Muscle tissue RNA yields very low and the quality...
11 January 2022 6,608 3 View
Hi All, I have many breast tissue samples and have stained them with two chromogens (Red and Yellow) and there is co-localization areas (orange) that I am interested to quantify as well. What...
11 January 2022 2,904 3 View
Hello! Right now I am struggling to perform a western blot with plasma-derived human exosomes. I want to confirm the presence of typical exosomal markers, e.g. TSG101, CD63 and CD9. I know that I...
11 January 2022 1,658 0 View
I'm trying to run an assay for Caspase 3/7 activity to visualize apoptotic cells in flash frozen, unfixed mouse skin sections. The assay involves a non-fluorescent reagent that is processed into...
10 January 2022 4,825 2 View
Hi, I am looking for chemicals or methods to solve plant tissue completely. Are there any specific chemicals or steps to do that?
07 January 2022 6,166 3 View
Is there any manual method for RNA later removal from tissue sample prior to RNA extraction?
06 January 2022 8,592 3 View
It is seen in recent years that during nanoparticle or single-atom catalyst system, people adopt freeze drying rather than drying keeping in a vacuum oven (where usually temperature is set 60-80...
06 January 2022 9,529 4 View
I have cubic PLA scaffolds with 1% bioglass (8 mm edge length, 400 µm pore size, 50 % porosity) containing fixed cells. I want to embed them in the mentioned resins for further cutting...
04 January 2022 4,702 1 View
I haven't found a protocol or any evidence if someone has used it for immunohistochemistry/immunofluorescent tissue staining. In the technical sheet it says it binds citrulline at acid pH, but no...
03 January 2022 5,429 1 View
Good evening please I need help, I am working on the reduction of hygrothermal models using proper generalized decomposition. I would like to have a document that explains this method
03 January 2022 273 2 View
All researchers I had a small query regarding the solubility of 2,4 dinitrophenyl hydrazine is soluble in water or not.
03 January 2022 4,236 5 View
Hi! I have an idea to use a chicken embryo as a model for endothelial disfunction studies. Who has already worked with the vessels of the embryo. It looks so beautifull and easily accessible...
30 December 2021 2,154 2 View
I am working on complement fixation test and having some trouble in sensitization of RBC's with amboceptors. I have to sensitized sheep erythrocytes in advance of performing titration of guinea...
28 December 2021 4,670 2 View
I am an international medical graduate looking to contribute to neurosurgery papers. My goal is to apply for a neurosurgery residency in 2024 and I would like to have plenty of papers published by...
23 December 2021 4,903 4 View
I'm running out of sheath fluid for my Fortessa and the vendor is also back-ordered. Can I make my own? Any tips to last me through the holidays?
23 December 2021 9,254 1 View