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Questions related to Tissue
Hi, I have a general question about pathway analysis in scRNAseq. I'm under the impression that scientists pick pathways relevant to their study from the list of GO terms and present those in a...
14 February 2024 8,511 3 View
Hello everyone I am trying to design an MTT experiment for an immortal adherent cell line with doubling time of 20 hours. The goal is to evaluate effects of certain growth factors and their...
14 February 2024 3,144 4 View
We are about to initiate #scRNAseq on some T-cell types. Back to literature, I found a wide diversity in the counts of the anlaysed cells used for the method (1K - 25K cells). I would really be...
14 February 2024 8,932 2 View
I I have RNA-Seq data for A. thaliana and I would like to perform clustering based on accessions to observe grouping patterns. What steps should I take? Should I consider interactome,...
13 February 2024 7,438 6 View
I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...
13 February 2024 7,687 1 View
Can the synthesis process for the following compounds be derived from sodium silicate and tetraethyl orthosilicate (TEOS)? 1. Methyltrichlorosilane (Me-Si-Cl₃) 2. Diethoxy(methyl)sodium silicate...
11 February 2024 3,511 3 View
I am working on identification of copy number in a recombinant Chinese hamster cell line by southern hybridization. I am having issues with the transfer of DNA onto positively charged nylon...
09 February 2024 2,624 8 View
I dissociated EBs and replated hiPS-CM into a monolayer in a fibronectin coated plates. I had some cells in 96-well plate and others in a 12-well plate. After 24 hrs., cells became adherent and...
08 February 2024 8,931 1 View
I am looking for recommendations for Kits for ECM protein analysis, preferably in a multi-well multi-target format. Trying to avoid mass spectrometry as it is expensive and complicated to do at...
08 February 2024 1,810 0 View
I am new to this forum and also new with using CST microwave studio. I have a question. When calculating port extension coefficient, sometimes I got minimum and maximum value for K, why this...
07 February 2024 573 1 View
The development of this research question arises from a critical review of the study conducted by Oguzhan Zengi on the transition from gel separator serum tubes to gel separator lithium...
06 February 2024 6,662 1 View
The subject of the study centers on the switch from gel separatory tubes to lithium heparin gel tubes in the clinical laboratory but the study additionally addressed the use of the Barricor...
06 February 2024 5,429 1 View
After Gram Staining, culturing is the second step in identifying causative agents which is time consuming and prone to errors.
04 February 2024 8,756 4 View
I have been trying to infect primary HFF-1 cells with Human Cytomegalovirus WT. Most of teh times the infection itself works. SOme of teh attempts, the cells looked very stressed after...
01 February 2024 2,686 0 View
How is thermal energy in the ocean transferred around the globe and heat transferred from tropical regions to Polar Regions?
30 January 2024 8,990 2 View
I have transiently transfected 67NRs, seeded in a 96 well plate at a seeding density of 15000 per well, using Lipofectamine 3000. 24 hours after transfection, I observed that there are atleast 50%...
30 January 2024 9,356 0 View
We are trying to stain cholangiocytes with mitotracker green and perform confocal live imaging. However, once we added mitotracker, the entire monolayer of cells started to peal off from the...
28 January 2024 2,660 1 View
Is there any guideline/consideration of the minimum Crystallinity Index value (from XRD) for a synthesized crystalline iron oxide? Thank you
27 January 2024 3,233 2 View
Aslamo alikom/ Greetings everyone, I'm conducting a western blot experiment (8% SDS gel) and I wanna test 2 proteins, one is 95 KDa, and another is 35KDa. The antibodies I've for both are mouse...
26 January 2024 7,616 2 View
If the mass of ny electrode is 3 mg and the surface area is 1 cm2, can i say my electrode's active mass is 0.003 g/cm2?
25 January 2024 2,154 2 View
Refer to uploaded video. When using serial EM in low dose mode the beam is moving when switching between focus and record. This results in bad images that are blurry. Anybody encounter this...
25 January 2024 713 2 View
Hi guys, We introduce some mice with two adjacent point mutation of specific molecules, however, it is difficult to carry out genotyping of this mice. DNA base pair and protein size is similar...
25 January 2024 9,938 1 View
Please suggest me a fast publication time journal related to cell biology(cellular Neuroscience), as i am a fresh phd student so have no idea?.. As I am fresh PhD student and I am not much...
25 January 2024 9,081 5 View
Hi, i am working on unnatural Amino Acid (UAAs) incorporated Insulin receptor substrate (IRS-1) expression and purification in BL21(DE3) strain. While i expressed my protein with UAA Glutathion...
24 January 2024 1,361 4 View