Recommendation for HNF1B ab for tissue IF application ?

31 March 2025 1 242 Report

Hello everyone,

I am looking for a reliable antibody for detecting human HNF1B expression in mouse kidney with immunofluorescence.

Dear Elena, if you require an antibody for detecting HNF1B in tissues via immunofluorescence, you're welcome to utilize MCE's recombinant antibody expression service to customize your ideal antibody!

Simply fill out your requirements at the link below. Our professional R&D team will promptly evaluate and respond to your needs.

https://www.medchemexpress.com/recombinant-antibody-expression-service.html

Hope this helpful and feel free to reach out for more details.

Badges
Science topic

More Elena Zion's questions See All

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Flow cytometry compensation with beads - is the matrix the problem?

I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...

07 July 2024 5,614 5 View

Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

How do you culture THP1 Leukemic Stem Cells?

Hi! I have to test a drug on the LSC fraction of THP1, but I'm not finding any specific media to select and culture them. Do you know anyone? Thank you in advance

15 May 2024 1,464 3 View

Methanesulfonic acid neutralisation?

Hello, I am performing esterification reactions between fatty acids and alcohols, in presence of methanesulfonic acid and H3PO2 as catalysts. At the end of the reaction, to reach the acid index I...

22 April 2024 2,063 5 View

Method DIN EN 14110 ?

I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena

22 April 2024 387 0 View

What is the last resort washing solution for a pepsin column that has a blockage?

Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...

20 April 2024 3,135 5 View

Why heptane is integrated in GC chromatograms ?

I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the...

07 April 2024 1,726 4 View

How can I calculate p-value from post hoc test for Kruskal-Wallis multiple comparisons in SAS?

I need to perform non-parametric post hoc multiple comparisons. I found two interesting macros that implemented methodology about post hoc test for Kruskal Wallis test, but p-value was never...

07 April 2024 288 3 View

What is the recommended sample size for the validation of a standard questionnaire consisting of 40 items?

Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...

26 March 2024 1,660 11 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View