Hi dear All. I'd like to know simplest protocol to detect ATP binding assay (ATP binding with protein). I read a couple of research about this but I want to know common methods. Thanks for your attention
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Hi everybody, I am setting up a method for analysis of colistin sulphate by HPLC-UV. I tried different previously-published methods but the results were not acceptable (long run time, bad...
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Hello everyone In the antioxidant method of FRAP-TPPZ, what is the ratio of the sample to the frap reagent solution? Different articles express different ratios. For example, 1 to 14 or 1 to 30...
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Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,639 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,658 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,272 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...
27 December 2023 8,915 2 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
29 November 2023 6,061 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
27 November 2023 7,979 1 View
process of human obtaining energy in cellular respiration
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I’m having issues with the stability of ATP. My lab has purchased multiple brands of ATP (Sigma # A6419; SantaCruz #SC-202040), each of which seem to come hydrolyzed. My lab uses the Malachite...
18 October 2023 3,648 0 View
Hi, all I am new to umbrella sampling, I have the done simulation of 2 gold nanoparticles coated with ligand and interaction with 2 different groups: Case 1: with atp Case 2: water But my PMF...
12 October 2023 4,666 0 View