How to solve a problem in analysis of colistin sulphate using HPLC-UV?

Hi everybody, I am setting up a method for analysis of colistin sulphate by HPLC-UV. I tried different previously-published methods but the results were not acceptable (long run time, bad...

16 January 2021 5,911 3 View

Does hyaluronic acid aggregate in the pH range of 3-4?

Dear researchers and Scholars Is it possible for aqueous hyaluronic acid solution to aggregate when entering to the colloidal nanosolution with pH about 3-4 ? I want to add hyaluronic acid to coat...

09 September 2020 9,688 3 View

How to get stable polymeric micelle?

03 July 2020 8,476 2 View

Why absorbance of dye solution increases after long irradiation time?

I have observed that the absorbance is increasing at long irradiation time during photocatalytic experiment for a perovskite material .Even I completely separate the catalyst particles with the...

13 December 2019 3,911 5 View

Has anyone ever used FCN (deeplearning) for one dimensional data in structural damage detection?

I'm working on deep learning in structural damage detection on bridge by signal (that is one dimensional data) . for that i want to get some information about Fully Convolutional Networks (FCN) in...

13 November 2019 9,347 3 View

How can I restore the powder used in Acid - Base Titrations ?

Working on a membrane for fuel cells ,after measuring the IEC by Acid (0.05 Hcl) -Base (0.05 NaOH) Titrations and using phenolphthalene as an indicator. We need to restore the powder.

03 October 2019 8,790 1 View

Connected component label in matlab?

I want to connect these labels .can I just connect these without converting it to bw ? and I need help in the algorithm of connecting these labels in matlab.Thank you

19 July 2019 7,777 5 View

Parsing of building facade in image processing?

Hi, I am trying to to have a segmentation of building facade just like these two pictures that I have sent.I have tried watershed algorithm but unfortunately it did not work. I mean I could not...

20 June 2019 9,511 3 View

Hi, i am looking for anionic polyacrylamide or poly(acrylic acid) with charge density around 2 meq/g and low mw of 50000 g/mol?

what chemical suppliers are providing such polymers?

09 September 2018 7,331 1 View

How to generate retroviral producer cell line using big construct?

Hi there, I want to generate retroviral producer cell line using Phoenix eco cells for transfection and E86 cells for transduction. My construct is about 9.2 kb but the insert part (from 3' of...

11 June 2018 6,842 6 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

ATP content measurement?

Hello, I am looking for a clear protocol for ATP content assay. I am not looking for a kit because it is expensive. I am looking for a fluorogenic or a colorimetric substrate that helps me to...

24 March 2020 2,286 3 View

ATP evoked calcium response in skin fibroblasts: no response?

23 March 2020 4,854 2 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

How to measure Km for ATP with Kinase Glo Plus?

Dear colleagues, Is there anyone who worked with Kinase Glo Plus reagent? I have to measure Km for ATP for ATP-hydrolyzing enzyme in the range from 0.3 to 100 mcM. Is it possible to do it...

11 December 2019 9,661 2 View