Standard Curve on elisa works but samples are negative?

21 November 2023 1 4K Report

I ran an ELISA using goat serum and after taking the readings the standard curve worked and looks good but serum sample wells are negative when the blank is subtracted. The serum samples actually read lower than the blank somehow

Demos Kynigopoulos

Your sample concentration is too low. Do a lower dilution of your samples

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