Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

Anyone with experience in virome sequencing using Oxford nanopore?

I am interested in sequencing the phages present in environmental samples (bioreators, seawater, soil...) using long reads from Oxford Nanopore sequencing. I would like to know if anyone has been...

06 April 2020 2,972 1 View

Is it possible to obtain information about the position of the atoms, taking into account the curves obtained by EDS?

In doped semiconductor samples it is very interesting to know the position of these atoms, if it is interstitial, substitutional, etc.

28 January 2020 845 3 View

How to calibrate a Pitot tube for a boundary layer wind tunnel?

Could someone please recommend me how to calibrate a differential pressure sensor for a pitot tube that will be used in a boundary layer wind tunnel, I bought a Pitot tube 160E Dwyer and a...

03 December 2019 933 3 View

R package: Pseudo r^2 in mixed effect model: which is the best approach and how interpretate?

Dear all, I am using linear mixed effect models in my experiment (using r package). I would like to show a measurement of the fitting quality of the model. I see that there are lot of methods but...

07 April 2019 1,686 11 View

ResearchGate RG score seems a bit confusing, someone said. Do you agree?

After adding more than 120 new research items (most of them, conference papers, and research papers published in Spanish Journals without online version, not automatically detected by Researchgate...

02 March 2018 5,685 0 View

What are the stages in formulating a agricultural waste management plan?

My project is about cocoa waste, I want to know both the diagnosis and the formulation of a management plan that can be applied

16 February 2018 1,691 9 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View