After activating the Staphylococcus aureus strain after 16 hours in TSB, I need to inoculate Mueller-Hinton agar plate with a volume of 0.1mL (concentration is 108 CFU/mL) for the Kirby-Bauer test. So how can I achieve a cell concentration of 108 CFU/mL using the dilution method, after activating them?
Thanks a lot.