Hi,
I'm new to flow cytometry. Basically, I measured immune cells in the heart using the BD device. And I found sometimes the range of threshold rate was not similar among samples.
For example, I prepared some samples at the same time. When I measured I found the threshold rate was around 12000 evt/s in some samples, but it could reduce to 5000 evt/s in others, which meant I would spend a lot of time on these samples. I also washed and cleaned during measuring. And although I measured the first sample, it was still around 5000 evt/s. So I don't think the order could be the reason.
I tried to find some information but I failed. I was wondering what affected the threshold rate. Why were the differences so huge among those samples?
Many thanks.
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