I would like to add an epitope tag to my protein (internal, not C- or N-terminal) for imunocytochemical labelling. Do you have suggestions for specific epitopes / antibodies that may work well, apart from standard ones (HA,c-Myc,V5 etc)?
As a stable element
23 July 2024 4,187 1 View
Hello, dear RG community. Personally, I have found Xarray to be excruciatingly slow, especially for big datasets and nonstandard operations (like a custom filtering function). The only suggestion...
15 July 2024 4,705 2 View
o impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?
10 July 2024 3,430 0 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you
29 May 2024 6,389 2 View
Hello everyone, I am currently exploring several options to give the collected data the greatest value possible. I have demographic data on older people, where I perform various memory and mood...
02 May 2024 876 6 View
Could you please provide me some authoritetic refers where this formula used if it accurate OR tell me why it is wrong and how to fix it? 1. TOA (L) = ML * Qcal + AL 2. BT = (K2 / (ln (K1 / L) +...
19 April 2024 8,924 0 View
Hello, dear RG community. Very often, gas fraction (also known as void fraction or gas holdup) is thought of as a ratio of the gas volume to the total volume:...
04 April 2024 7,290 0 View
Dear Researchers, I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells. My aim is to engage with fellow...
22 March 2024 4,438 0 View
I'm researching about videogames and their effect on metacognition, actually I intend to develop a game with that specific intention. I'm tinkering with a puzzle game that gives feedback and maybe...
18 March 2024 1,147 2 View
Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...
12 June 2024 7,332 0 View
I recently developed a few mAbs that are able to neutralize SARS2. By ELISA and WB, I found that they all bind to SARS2 RBD. I wanted to map their RBD epitopes so I ordered pre-coated plates with...
02 June 2024 2,877 2 View
i want to use pepfold for epitopes simulation and docking but im not quite familiar with pepfold or docking in the first place, im asking about a clear guide if possible for the section of docking...
16 April 2024 659 0 View
I'm performing some DAB-enhanced anti-GFAP staining. My primary is polyclonal chicken anti-GFAP (AB5541): The antibody isotype is described as N/A in the SDS. We have a goat anti-chicken IgY...
14 April 2024 9,715 1 View
Either the epitope mapping software or an expert to collaborate with in the epitope mapping. Better still, a design of the mapping software in case none exists.
08 April 2024 9,102 0 View
The Plasmodium DNA sequence will be provided if needed. Alternatively, the mapping instrument that can be able to predict potential epitopes within the DNA be provided for me to work with.
05 April 2024 6,968 0 View
Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...
28 March 2024 259 3 View
I have tried 4 IBA1 antibodies and cannot seem to get good staining. Mice were perfused/fixed with PBS/4%PFA. Brains were placed in PFA overnight, and then moved to 30% sucrose and frozen in OCT...
19 February 2024 2,835 5 View
I have not found any anti-iGluSnFR antibodies available, so I am curious if standard anti-GFP antibodies could recognize some epitope in the circularly permuted form of GFP in glutmate sensor...
04 February 2024 5,824 2 View
Hi, I am trying to customize a TR FRET kit for the specific analyte that I need to detect. For that I am planning to use, an antibody labeled with Terbium (donor) and the other antibody labeled...
16 January 2024 9,981 2 View