I did antifungal disc diffusion test on Candida albicans and even I have repeated this test many time. However, the control positive (fluconazole) gave negative result. I used newly bought antibiotics disc, but the result was still negative. I used PDA for this test, incubate at 37 degree celsius for 24 hours. This is my first time the result turned out to be like this. Does it means that the antibiotic already resistant? Or should I shorten the time of incubation? Or maybe change of the agar? Need opinion from the expert very urgent. Thanks in advance.
Most likely your C. albicans strain is resistant to fluconazole. You can try the same in a different plate with a strain that you know for sure to be sensitive to fluconazole.
Perhaps these are resistant isolates. I conducted a test on Candida albicans, some of which gave a positive result, some negative, and also on PDA medium, and the time was between 23-48 hours.
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