Photoionisation cross section of (singly) excited Helium?

Hello, I am searching the photoionisation cross section of (singly) excited Helium (1s2s as well as 1s3s). I only find either neutral Helium or doubly excited Helium. If someone could provide...

17 November 2020 7,096 3 View

Problems with PSD-95 IHC in 30um brain slices?

I'm optimising the PSD-95 antibody 7E3 (Santa Cruz Biotechnology) to stain some 30um brain slices (resectioned from 300um). The current protocol I'm using has not been very successful in that I'm...

14 December 2019 8,937 1 View

Is anyone aware of a report that assesses healthcare professionals attitudes/ opinions towards artificial intelligence/ machine learning?

Hi, I am looking for a report that assessed healthcare professionals' opinion/ attitudes towards machine learning/ artificial intelligence; preferably with a large sample. Any recommendations...

18 January 2018 8,852 3 View

Has fluent been compared to starccm+?

11 September 2017 9,674 5 View

Does anyone know how I can conduct a moderation analysis using spss wherein my IV has 4 dimensions, a categorical moderator and 1 DV?

I have read and watched videos about moderation analysis/interaction using spss. however, only provides 1 DV, 1 IV and moderator. My paper aims to know if dimensions of IV can predict DV and if...

22 December 2016 885 4 View

WPBR & Great Basin Bristlecone Pine (Pinus longaeva)

I am writing my dissertation on WPBR in the Northeast and am wondering if the disease has yet been found on P. longaeva, confirming the presence of the disease on all native white pines in North...

01 January 1970 4,347 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View