The best thermal imagers in zoological research for detecting small mammals in soil?

What are the best thermal imagers to use in the study of insectivores, rodents and carnivorous mammals in soil horizons? The ability to detect herpetocomplex with a thermal imager in natural shelters?

17 November 2020 346 5 View

The best portable recorders for studying temperature changes in snowpacks?

What are the best loggers for obtaining changes in snow cover temperature at different depths with changes in air temperature? What are the long-term data loggers without recharging? What loggers...

17 November 2020 5,319 5 View

Suitable channel model and beamforming technique for high band 5G?

Hello everyone, I want to simulate traffic in MATLAB between a multiple antenna base station and a single antenna user for mm-wave frequency of 28GHz. What is the most suitable channel model for...

20 March 2020 8,156 4 View

I want to use a Dialetric resonator antenna to measure magnetic fields, but how can I reduce the ambient noise from the environment?

I'm working on a project to do sense magnetic fields in 100 nano tesla range, and I don't know how we could reduce ambient noise without shielding. - Artificial magnetic fields could be one way...

03 July 2019 8,808 3 View

How to export chromatograms and spectra from diode array detector data?

The DAD data are collected into a .run file during an HPLC run followed by a Varian Prostar 335 detector. The file cannot be merely opened via a notepad and the question is how to extract all the...

04 April 2019 9,649 3 View

Why can a protein elute from a SEC column (@150 mM NaCl) later than expected for its monomer?

Theoretical mass is 25 kDa, SDS-PAGE shows 26 kDa, no presence of degradation products. The SEC profile shows the major, very symmetrical peak at ~20 kDa. Why could this be? Unspecific interaction...

28 August 2016 2,007 16 View

Why do we need a "Smart Robot" to be used for recipes development and controlling performance?

We are discussing here intensive quenching processes: IQ-2 and IQ-3 technologies. The IQ-2 technology requires the duration of transient nucleate boiling process evaluation during batch quenching....

07 July 2015 6,143 5 View

What are the main differences between steady - state and transient nucleate boiling processes?

I'm wondering whether we can use heat transfer coefficients (HTCs) received for steady - state nucleate boiling condition to calculate temperature fields in steel parts during transient nucleate...

28 January 2015 1,475 11 View

Why engineers instead of real HTCs use effective HTCs during boiling?

17 December 2014 7,955 4 View

What is the original/up-to-date technique used for intensifying quenching of machine components based on transient nucleate boiling processes?

In metallurgy the effective heat transfer coefficients (HTCs) of transient nucleate boiling processes are used which are ten times less as compared with the real HTCs. The original technique for...

11 October 2014 2,050 2 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View