Why there is cytoplasmic stain of KI67 in HCC mouse liver tissue?

15 November 2023 1 157 Report

When stained with KI67 in mouse liver tissue with HCC, I can see cytoplasm stained and not nuclear staining. This is IF staining where the tissue is fixed with formalin and permeabilized with 0.2% triton x-100.

Alexander Shtabsky

You are describing non-specific cytoplasmic binding. I'd consider either IF in FS or chromogenic IHC (peroxidase, phosphatase) in FFPE

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