I was looking for nucleic acid detection with CRISPR. I wish to know how and where to design the crRNA? Which company deals with it? Any lead would be appreciated.
https://www.neb.com/en/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a
Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...
07 July 2024 3,526 3 View
we need surface area, corrosion current, equivalent weight and density of the metal involved for corrosion rate calculation in mm/year (lets say). For dissimilar metal welds, how can we measure...
30 June 2024 5,943 2 View
Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...
29 June 2024 10,023 1 View
I do a research about Non-Verbal Communication: Gestures and Body Language in Classroom.
18 June 2024 8,737 1 View
In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...
17 June 2024 3,888 2 View
Action research design is Quantitative Studies or Qualitative Studies?
14 June 2024 1,684 1 View
Hi there! I was wondering how I should go about interpreting the following data. I am currently investigating the moderating role of working memory (continuous) on the relationship between...
07 June 2024 1,473 0 View
Is there anyone to tell me the scientific name of this mushroom?
03 June 2024 464 5 View
How can we simulate TSV in the Silvaco example? Exactly, I need the instructions for the attachments file. Best regards.
29 May 2024 1,638 1 View
best firm level database for south Asian firms?
28 May 2024 3,424 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View