I was looking for nucleic acid detection with CRISPR. I wish to know how and where to design the crRNA? Which company deals with it? Any lead would be appreciated.
https://www.neb.com/en/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a
I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...
13 February 2021 6,410 3 View
In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...
11 February 2021 4,006 8 View
Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell. And he talked about one of them?
09 February 2021 1,099 8 View
While reviewing technical articles, I encounter some papers where authors have used data balancing before splitting test and train (on full data), and some only balanced training data and let the...
04 February 2021 1,446 0 View
severity index, measuring method and procedure detailed
01 February 2021 1,160 11 View
The 1st image shows the moment when I import my design(IGES) into the Mechanical APDL. The 2nd image occurs when I wanted to Operate>Boolean>Divide Volume By Area. The design is a regular...
28 January 2021 2,962 3 View
Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...
18 January 2021 9,045 3 View
Why there is no licensed vaccine available against congo fever? Besides what should be done to make the globally recognized Congo Vaccine?
11 January 2021 4,475 4 View
Every function of mind works in the brain. Study of divisions of mind for memory, thinking, analysis, conscious and subconscious mind is important for successful use of the mind.
10 January 2021 5,282 32 View
How to obtain creep parameters for running Norton Bailey model, material SS-304 ?
06 January 2021 8,074 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...
24 February 2021 4,578 4 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
22 February 2021 215 2 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View