What does a negative real part of impedance indicate in a Nyquist plot?

I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...

12 January 2021 7,399 14 View

How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.

01 December 2020 1,099 2 View

What is the difference between the near field and the far field effects in optics?

A simple definition might help to understand the diffraction pattern in an aperiodic nano-micro structured surface.

19 July 2018 3,865 6 View

Is there a way to attach a nano particle to the cell membrane and prevent it from entering the cell?

01 June 2016 5,159 3 View

Will RGD coated nanoparticles be internalised by cells?

24 December 2015 6,303 4 View

Do we have "mesenchymal" breast cancer cell lines?

24 November 2015 8,669 6 View

What is the difference between various breast cancer cell lines?

24 November 2015 8,487 5 View

Is there any correlation between substrate stiffness and substrate roughness?

Can tuning roughness leads to different stiffness of a material?

18 October 2015 3,286 8 View

Is flow cytometry capable of calculating the viability rate of a low viscous cell solution?

As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether...

22 February 2013 5,130 2 View

Dual-fluorescence viability VS Trypan blue, which one is more accurate and why am I observing such a distinctive difference?

While encapsulating ADMSCs in sodium alginate, providing a 3D environment for the cells, I come across different viability rates. Trypan blue shows a lower viability rate, as low as 50%. On the...

19 February 2013 6,200 5 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

How to analysis orientation molecule adsorbed on mineral clay molecular dynamics using gromacs?

I'm still confused. Whether to determine the methylene blue orientation, I have to use the order parameter (gmx order) or the gmx angle? I want to determine the orientation of the methylene blue...

31 December 2020 2,386 3 View

How to compare two images obtained from optical microscope?

What program can I use to evaluate two images from an optical microscope, e.g., before and after methylene blue staining. I would like to quantify the degree of staining of cells with a dye....

14 December 2020 9,223 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Blue native page transfer problem?

Hello, I am writing this hoping someone can help me. I am just starting out with BN-PAGE and I have a transfer problem. It seems each time I transfer (2 membranes at a time) in a BioRad wet...

09 December 2020 1,008 3 View

Is there any possible stabilize metal-doped blue-emitting light perovskite through self-assembly method?

Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...

04 November 2020 8,384 3 View

BBB permeability assay in mice ?

Hello! Is there anyone who could recommend a protocol for testing the permeability of the blood brain barrier in mice ? I tried to use these protocols (Assessment of Permeability in Barrier Type...

01 November 2020 8,191 3 View

What are the protocols for H&E Alcian Blue method?

i want to know the protocols for H&E Alcian blue method for studying histology of intestine of fish

26 October 2020 6,351 3 View