Does anyone have tips for expanding a vector from a supplier? It's ~5ng/uL and stored in 50% glycerol. I attempted to do a transformation as normal but I did not get any growth.
If it is small you can amplify it using PCR.
I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...
09 February 2021 4,866 3 View
We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...
16 January 2021 4,791 2 View
Hello, I'm doing a research regarding gas adsorption on hydrophoobic biochar. Is there any way to coat PDMS on the biochar powder?
05 October 2020 5,610 1 View
Hi, I'm currently looking for auditory stimuli (list of letters; word/non-words; etc) to include as distraction task in my experiment. Does anybody know a free database that I might download?...
04 October 2020 9,614 3 View
Hello people! I would like to understand if oncologists and doctors in general would be interested and indicate treatment for bone metastasis originating in breast cancer, ER +, PR +, HER-2 - with...
14 September 2019 4,322 3 View
How do I determine whether a new measure I'm developing is "more valid" than existing measures of the same construct? I'm proposing to develop a new measure that is more inclusive of the...
15 May 2019 9,893 4 View
I'm having an issue with non specific binding of my primers. I've tried many primers and this is the least messy primer pair but I am still getting a second melt curve peak when doing serial...
19 September 2018 3,940 3 View
I am planning an experiment using TTX on C2C12 myotubes. The TTX should be reconstituted in citrate buffer pH4.8. I am now trying to find out which molarity of citrate buffer can be used for C2C12...
05 July 2018 2,754 1 View
Hello everyone, I really need your help! I am doing my bachelor thesis and I am struggling with the following. I want to do moderation analysis with the following variables: - IV: Collaboration...
11 June 2018 9,607 7 View
I'm looking for a good physiological model of the lungs (and thorax), to do some non invasive monitoring of lung function parameters
09 January 2018 6,744 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
Covid 19 Pandemic and digital transformation
22 February 2021 3,351 5 View
I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.
21 February 2021 3,081 3 View
For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View
1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...
20 February 2021 2,370 1 View
i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?
19 February 2021 2,352 5 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...
15 February 2021 5,655 14 View