Does anyone have tips for expanding a vector from a supplier?

19 October 2023 1 1K Report

Does anyone have tips for expanding a vector from a supplier? It's ~5ng/uL and stored in 50% glycerol. I attempted to do a transformation as normal but I did not get any growth.

Robert Adolf Brinzer

If it is small you can amplify it using PCR.

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Science method

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