Could you please tell me which sequence should be chosen when a sequence is alligned on blast and both have the same score, E-value and ident?

More Ravi Luckhun's questions See All

Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

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HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

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Yeast Cell Glucose Uptake Assay ? Can yeast release more glucose into the reaction, when treated with test compound ?

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02 April 2024 7,657 2 View

Suggest good geospatial data security papers ?

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12 March 2024 4,917 2 View

What is the difference between the single microgrid and multi microgrid? why we consider Multi microgrid in an area but not as a single microgrid ?

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03 March 2024 9,306 1 View

Wire bonding issue (Au/Ti/GaN). Any feasible solutions please?

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How to calculate the precision for a RT PCR assay?

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11 December 2023 1,812 1 View

How to add my articles in research gate?

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22 November 2023 5,926 3 View

Statins causes liver damage upon prolonged use. To control it, given natural with hepatoprotective activity. But results HDI! what might be the cause?

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09 November 2023 587 0 View

Why monocytes adhere while differentiating them as Dendritic cells?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

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Are the apoptotic cells is positive for γH2AX ?

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Sabrina D Dyall

Hi Ravi,

If you are DNA barcoding, you would have clearer results if you do the BLAST within the BOLD system at http://www.boldsystems.org/

The sequences in BOLD have been curated and you can also generate a distance tree rapidly for an indication of the identity of your test sample.

Ravi Luckhun

Dear Friends, Thank you very much got the information which has helped tremendously in the research.

Best regards