AmCube/FootWorksPro data extraction?

Has anyone got any experience of using the Amcube pressure plate with Footworks Pro software? I have a load of static standing balance measurements that the software provides (movement of centre...

27 December 2020 7,161 1 View

Can you recommend a task-based material that can be used to compare with the currently used textbooks in English teaching?

Hello everyone, It was my first experience for a research. I plan to explore the differences in grammar teaching in the two kinds of books: one is task-based and one is currently used in a...

29 October 2020 5,495 4 View

Any difference in model selection between SAS and R?

Hello all, We are working on a project as a team and some of us using R and I am using SAS. For some reason, we are getting different best models using forward and stepwise methods. I am getting...

10 November 2018 7,179 1 View

Minimum clinically important difference (MCID) and sample size calculation for interventional studies

Hi all, Would you please advise me how to use the "minimum clinically important difference - MCID" to calculate sample size for interventional studies? Are there any web based sample size...

18 May 2017 4,868 8 View

Is it right to calculate O/C ratio from deconvolved peak areas of C1s peak?

I am performing graphene oxide reduction on SiO2 substrate. In this case, the XPS spectra will measure C1s, O1s and Si2p. Due to the O in SiO2, the O/C ratio cannot be simply calculated from C1s...

08 February 2017 7,301 6 View

How can I validate the purity of GST-tagged protein?

18 December 2016 1,769 9 View

Is there any paper about calculating mass loss of reinforcements in steel fiber reinforcement concrete by corrosion current density?

My research is about the effect of steel fiber reinforced concrete with transitional reinforcements. I could only find the relationship between corrosion current density and mass loss of...

11 December 2016 7,554 0 View

Does anyone have idea why shRNA and SiRNA show opposit effects on immune response on RAW264.7 cells?

Hello everybody, I am trying to produce ShRNA stable cell on RAW264.7. I succeed to get cells which is around 70-80% knockdown in puromycin selection condition. But the thing is when I measure the...

22 May 2016 7,188 6 View

Culturing EOL-1 cell line (a human eosinophil cell line)?

Dear all, I would like to ask for your advice with culturing culture Eol-1 cells (eosinophilic cell line). I resuscitated cells from frozen stock and suspended in RMPI 1640+ 10% FBS + 1% PS. After...

01 May 2016 6,653 8 View

Is it normal to have the immunofluorescent staining result like this? What's possibly wrong with it?

I used formalin-fixed paraffin-embedded liver tissue to do the immuofluorescent staining, but the result doesn't look good, can anyone tell me what is possibly wrong with it? I used 20% normal...

06 April 2016 942 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View