I am trying to amplify an AT rich sequence which is 1543 bp long but still unsuccessful. I have used Phanta Max and Taq enzymes. Please suggest possible solution for it.
What other conditions have you tried varying? Within a PCR reaction there are multiple variables other than just the enzyme used.
For example, have you tired altering primer set, annealing temperature, extension temperature, MgCl2 concentration, DMSO, No. of cycles etc?
I personally have always used Q5 or Vent polymerases from NEB and with some optimisation get the amplified sequence required. hope that helps?
I agree with Simon; we built entire Mycoplasma mycoides genome (GC ~20%) using Phusion/Q5 Taq polymerases. I'd definitely increase the MgCl2 concentration, it seems to help with most low GC amplifications. You could also try adding ET-SSB to your PCR reaction. Hope this helps.
I agree with previous responses. Other approach I have used for low G+C Bacillaceae species is Touchdown PCR, also it was helpful in avoiding non specific products due to low annealing temperatures.
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