hi
have you ever experienced of coming different results from western blot and icc?
I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I expected reduced expression of a-syn pathology (e.g. low level of phospho-a-syn or aggregation)
I observed reduced expression of p-syn in ICC, but the other way around in western blot
if anything, the amount of the a-syn aggregation was increased in experimental group along with western result
I am so confused which data I can trust and go for