26 May 2024 0 3K Report

hi

have you ever experienced of coming different results from western blot and icc?

I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I expected reduced expression of a-syn pathology (e.g. low level of phospho-a-syn or aggregation)

I observed reduced expression of p-syn in ICC, but the other way around in western blot

if anything, the amount of the a-syn aggregation was increased in experimental group along with western result

I am so confused which data I can trust and go for

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