Are those Strep-Tag II sepharose beads compatible w Glycine elution at pH:2.5?

More Gulden Yorgancioglu Budak's questions See All

Which order is right-1 oral presentation 2 manuscript; or 1 manuscript 2 oral presentation ?

I want to present my work as oral presentation in an International congress which they will publish the summary in an International journal. If I publish this study as an full text article in a...

22 June 2018 4,720 2 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

21 December 2016 8,975 6 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

11 December 2016 9,713 6 View

Hba1c result different between boronate affinity and HPLC method?

When comparing HBa1c analyzer in our laboratory we compared 120 patients results with each other? We performed the study in 2 same brand boranate affinity and two HPLC analyzer. All the patients...

21 March 2015 9,077 2 View

Which software is free and easier to use for RSM and CCD?

Do you have any suggestion, please share here. Thank you.

09 January 2015 1,769 20 View

How can one use TSP-PRED properly for submitting protein sequences?

I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.

15 December 2014 9,621 5 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

04 August 2013 4,036 11 View

How to trim adaptor / linker sequences from EST sequences before submitting to NCBI?

Any available software for this purpose?

29 July 2013 612 2 View

Does anyone have experience with protein precipitation from yeast cell lysate (Saccharomyces cerevisiae)?

Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...

23 July 2013 1,394 5 View

No migration of protein sample from stacking gel to separating gel

What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...

16 July 2013 3,342 27 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View