Which of the following proliferation-tracing dyes perform the best?

25 April 2024 0 3K Report

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to untreated controls. I'm using Tk6 cells, a lymphoblastoid cell line.

I am considering a dye-dilution method, with the quantification algorithms built into FlowJo.

The cells would be:

Stained with the dye then resuspended in growth media

Treated for up to 24 hours untreated cells are expected to undergo one or 2 cell divisions during this period, the cells are not synchronized so the population will be mixed.

Analyzed by flow, using FlowJo's proliferation model to estimate the number of cells in each division

I'm considering the following dyes, I was hoping someone could recommend which one might perform the best for my intended purpose or another dye that might outperform the ones I have listed.

BD Horizon Violet Proliferation Dye (VPD450)

BD Horizon CFSE

CellTrace Violet from ThermoFisher

CytoPainter Green Proliferation from abCam

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