Can any one provide with a recommended DAPI staining protocol for dental pulp stem cells already fixed and dried for SEM imaging?.The cells are fixed on dental chips 4*4mm.Want to use these samples for fluorescence imaging instead of SEM imaging now.
Howard Vindin
I'd just rehydrate with any suitable solvent (MeOH, EtOH, etc) and PBS and once it's in PBS DAPI stain in the usual manner. I'd suggest 1/10,000-20,000 for 10-15 mins followed by 3 x 5 minute washes with as much PBS as can fit into the well/container/whatever your staining it in.
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