Hello,
I don't have access to cryo SEM in my country and I have protein samples (Whey protein) that are sensible and are burnt by the bombard of electrons under SEM device.
Is there a possible solution that I can take good images via SEM/FESEM? For example, after fixing my solution by using
Glutaraldehyde.
Thanks
Just try to read this article [Rosenberg, M. and Lee, S. L. (1993) "Microstructure of Whey Protein/ Anhydrous Milkfat Emulsions," Food Structure: Vol. 12: No. 2, Article 14].
Dear Iman Katouzian,
Cryo-SEM :
Is a quick, reliable and effective way to over come these not inconsiderable SEM preparation problems. Additionally the technique is widely used for observing 'difficult' samples, such as those with greater beam sensitivity and of an unstable nature. An important application, often overlooked, is the ability to use cryo-SEM to study dynamic processes (industrial or otherwise) by using a series of time resolved samples.
SEM/FESEM :
The main difference between the SEM & FESEM is "Emitter type".
FESEM uses Field Emission Gun producing a cleaner image, less electrostatic distortions and spatial resolution < 2nm (3 or 6 times better than SEM).
I understand that you don't have access to cryo SEM. For better understanding, I have attached herewith the Research Article links which uses SEM, FESEM and also cryo-SEM.
01. Article Properties of partially denatured whey protein products 2: S...
02. Article Cleaning-in-Place of Whey Protein Fouling Deposits
03. Article Surface Properties of Aerated Ion-induced Whey Protein Gels
04. Article Cell envelope disruption of E. coli Exposed to ϵ-polylysine ...
05. Article The application of electron microscopy in dairy research
I hope I have answered your question.
With Best Wishes,
Samir G. Pandya
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