I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you managing this?
As an alternative, I was wondering if there's comercial Kits for Alkaline phosphatase conjugation. I don't have structure to produce it in e. coli, for example. I would try coupling a primary antibody to AKP with a kit. Anybody knows if exists something like it? Any ideas to help me having an anti-DIG-AKP antibody to keep my assays runing?