hi every one
I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with RNA extraction of Human fibroblast and PBMC (from Blood).
can you please help me how i can chosen the best source of this sequence for extraction of RNA?
thanks with best regards.
Satyendra Mondal
Hello, I did not understand your question well. Did you try to amplify the desired gene segment using RNA library as a template? Have you prepared the cDNA library from the extracted RNA sample? If not, then you are using wrong template for the PCR.
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