Greeting
How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?
Are 100 events count sufficient and three repetitions for analysis statistic ?
Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...
08 July 2024 4,724 4 View
Still alive but new info from last hospitalization a week ago. Per Endo ... Covid remapped the electrolyte panel. A high potassium 5.5 actually is low and the patient needs K+ Blood glucose at...
21 May 2024 4,780 0 View
The past few months I have started testing the selective androgen receptor modulator (SARM) GSK2881078 to study its ability to increase muscle growth and/or counteract muscle atrophy. I have done...
20 May 2024 8,747 0 View
Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide? What is the protocol?
16 April 2024 1,409 1 View
For validation by qPCR of several microRNAs, is it necessary to measure normalization miRNAs in each plate in addition to the miRNAs under study? Or can you make plates of microRNAs and then...
15 April 2024 6,393 1 View
Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...
25 March 2024 7,648 0 View
I need a little help, how do you make "Traits" in PopArt? I need to make a network of haplotypes on a map
14 March 2024 2,316 0 View
Hello, I wanted to ask for help with protein modeling programs (not servers) with a free commercial license. thank you
13 March 2024 6,173 0 View
I would to delete up to 33 bp from a plasmid to generate deletion mutation. Has anyone tried using Agilent Quikchange lightning site direct mutagenesis to delete such a long region? How feasible...
06 March 2024 8,136 5 View
I'm preparing liposomes by thin film evaporation method. I'm wondering how many water I sholud add during hydration step? The next step will be the extrusion using 100nm PC membrane.
05 March 2024 3,404 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View