How to model the causal effect of working part-time compared to full-time on health?

Hi there, I have to estimate the causal effect of part-time work compared to full-time work on health. I have an unbalanced panel data set with t=2008-2017, except for year 2009. The main issue...

11 December 2020 3,170 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View

Does anyone have any advice for Intracellular Staining of methyltransferase for flow cytometry?

Hi All, I have been attempting intracellular staining of a methyltransferase and have been running into issues with consistency. I have attempted sequential staining with a PE secondary and have...

20 November 2020 2,314 1 View

How many casual deaths constitute scientific evidence? Do we know the risks of repeating influenza vaccination?

Italy (2014). Around 35 death after and very closer to the flu vaccination Spain (2020). At least 45 death reported in correlation with adyuvate flu vaccination and 6 time risk to died with...

22 October 2020 2,746 2 View

Use of FcR blocking reagent for B cell surface IgG staining in flow cytometry?

I would like to measure cell surface IgG in human culture of B cells. I have culture of human peripheral blood mononuclear cells (PBMCs) that were immortalized with EBV (Epstein-Barr Virus) and...

30 August 2020 6,805 2 View

After incubating overnight in blocking buffer (BSA) at 4C, how long can i incubate in Primary antibody the following day?

Hello Everyone, I usually block my membrane in BSA blocking buffer for 1 hour before incubating in primary overnight. This time i was pressed for time and decided to block at 4C over night....

05 August 2020 7,007 4 View

Request for suggestion on bimonthly journals in industrial water pollution management ?

Can anyone suggest some bimonthly journals which are either Scopus or UGC indexed in the area industrial water pollution management? thank you?

24 July 2020 2,036 2 View

Supplier for NiTi powder?

28 June 2020 6,530 4 View

Which is the most recent phylyogenetic tree for rodents?

Dear all Does someone have a recent rodent phylogenetic tree (preferably in Nexus format or any other format usable in R) or knows a publication with a recent tree? Currently I am still using...

24 May 2020 3,620 4 View

Does anyone know a school offering a BioInformatics short course in Dallas?

I am finding it pretty difficult doing an online program. Does anyone know of any school offering a short program or continuing education in Bioinformatics?

27 February 2020 6,065 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View