How measue exosomes concentration ?

17 January 2025 1 5K Report

Dear all, we have particle/mL (by nanosight) how it is possible to know exosomes concentration (ug/mL)?

thanks

If you have access to flow cytometry equipment that can handle EVs (for example, Cytek Aurora), you can label your particles with antibodies against exosome markers (anti-CD63 or anti-CD9) conjugated with a Fluorescent dye and determine the percentage of the labeled particles.

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