Did anyone use LPS to stimulate L929s produce IFN?
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...
23 July 2024 6,127 1 View
This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...
09 July 2024 7,883 0 View
Using magnetron sputtering with an alloy target composed of A:B:C in a 2:1:1 ratio, the resulting thin film composition is A:B:C=1:1:1. How can you achieve a A:B:C=2:1:1 composition? What are some...
24 June 2024 1,605 0 View
Given the energy level diagram of a ruby laser shown in the right figure, where \( R_{02} \) is the pumping rate, and \( \tau_2 \) and \( \tau_1 \) are the lifetimes of levels \( n_2 \) and \( n_1...
23 June 2024 4,855 0 View
Can the matter wave theory, which suggests that when matter waves resonate, the energy input from the outside is minimal, be used to solve all superconductivity problems? The matter wave theory,...
17 June 2024 7,957 0 View
till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...
12 June 2024 1,908 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷
13 July 2024 3,382 0 View
I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...
07 July 2024 833 1 View
Hello, all I am currently extracting LPS from E. coli MG1655 and its mutant strains. I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see...
25 June 2024 7,568 1 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...
16 June 2024 928 1 View
I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...
16 June 2024 2,885 3 View
I have been using the Lonza nucleofector for RNP delivery to OT-I T cells without any issues for the past six months. However, recently, my nucleofected OT-I T cells have not been proliferating in...
13 June 2024 2,279 0 View
