Older FFPE tissues are used for RNA extraction by Trizol based method which is then transcribed into CDNA using a kit, having a lower concentration and purity. Thermo Scientific kit is used for this purpose. But the question arises on the cDNA being formed since data proposes that such RNA undergo fragmentation which reduces the efficiency of further down streaming processes. So which kit-based method might help in getting adequately synthesized cDNA to yield real time amplification product.