Proteomics to track/ trace origin of exosomes?

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CD31 band at 55 kDa?

I performed Western blot for CD31 marker with expectation to observe band at ~130 kDa. However, i observed bands at 55 kDa for all my samples. According to supplier, additional bands are usually...

14 November 2016 4,149 2 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

An optimal protocol of EVs isolation for proteomics?

Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...

17 December 2020 7,600 2 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View