Proteomics to track/ trace origin of exosomes?

More Nanthini Jayabalan's questions See All

Is it possible to administer drug dissolved in ethanol orally ?

I wanted to give Cholecaliciferol (vitamin D) dissolved in ethanol orally to Wistar rats aged 5 to 6 months. Majority of the research articles suggests Subcutaneous route however I would like to...

27 September 2021 9,689 3 View

CD31 band at 55 kDa?

I performed Western blot for CD31 marker with expectation to observe band at ~130 kDa. However, i observed bands at 55 kDa for all my samples. According to supplier, additional bands are usually...

14 November 2016 4,235 2 View

Specific marker for exosomes isolated from adipocytes?

Hello everyone. Im aware of exosome specific markers such as CD63. However, im wondering if there are any specific markers for exosomes isolated from adipocytes? Thank you in advance.

23 August 2016 7,603 2 View

Iodixanol gradient vs sucrose gradient

Can someone please enlighten me on why some prefer to use iodixanol gradient compared to sucrose gradient? Is this dependent on type of cell that is being used? Are there any major differences...

21 August 2016 9,557 3 View

Why the probability of electron density of 's' and 'd' orbitals is differ?

why the probability of electron density for 's' orbital is in spherical shape and for 'd' orbital not in spherical spherical shape?

16 August 2016 5,034 2 View

CCI or FPI to study the basal forebrain (BF)?

Hello everyone. We are intending to study the effect of TBI on BF neurons. I did some literature search and found both FPI and CCI model have been used for this purpose. Between these two models,...

07 June 2016 7,140 2 View

How to choose a solvent to dissolve a crystal for liquid crystal display?

we are trying to dissolve a crystal for liquid crystal display and would like to know which solvent to choose.

25 February 2016 275 2 View

Can any one provide thin section photographs of melilite bearing lamprophyre or alnoite?

We had encountered a small dike with evident porphyritic texture in both hand specimen and thin section scales. The euhedral minerals are colorless, tabular or elongated tabular some times wedge...

11 March 2015 1,188 32 View

Are there any (online or offline) tutorials for GCDkit scripting in 'r' language?

GCDkit is one of the most versatile packages for igneous petrology. Writing scripts in 'r' for GCDkit has a steep learning curve. Tutorials may help to facilitate learning with feedback.

10 July 2014 144 12 View

What is crustal delamination and how does it influence the petrogenesis of alkaline rocks, e.g. lamprophyres?

Analytical data of lamprophyres (minette and spessartite) display a primitive character and are enriched in LILE with depleted HFSE. The geotectonic environment as suggested by trace element data...

04 July 2014 2,508 8 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

About exosome isolation?

Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...

01 July 2024 2,558 0 View

My question is about a barley seed ?

I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.

26 June 2024 3,451 2 View

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

How can I optimize the protocol of pirfenidone loading into the exosomes (sonication or electroporation)?

What parameters are important in this process?

17 June 2024 4,463 0 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

Co-incubation of neutrophils with exosomes?

Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...

29 May 2024 6,551 0 View

How to handle missing data for proteomics data?

I have a proteomics dataset with missing values. I tried some strategies, but the point is that there are sets completely with missing values. The last strategy was to apply MissForest in python...

29 May 2024 5,563 4 View