Can I use a single polyclonal antibody batch split in two for a sandwich assay?

I have been using kits with paired monoclonal antibodies, what I wish to do is purchase polyclonal antibodies to a particular antigen and split in two, immobilise one half and fluoro label the...

31 December 2018 5,141 7 View

When measuring Zeta Potential in ethanol, is it necessary to add salt?

Hi, I'm new to this type of measurement, from what I've read, the addition of salt is vital in aqueous measurements of Z. However I have been measuring a silica micro-sphere suspension in pure...

06 July 2018 6,214 6 View

Can polyelectrolyte bi-layers be formed from organic solvent?

I have Raman functionalised Ag microspheres and wish to coat in polyelectrolyes, however when I transfer them to aqueous solvents for this it becomes far more difficult to wash the sample and I...

03 April 2018 3,942 0 View

What alternatives are there to using Piranha solution for cleaning silica before silanization?

I have SERS microsphere sensors that have been silica coated, I have been coating them in APTES for further protein crosslinking. In order to get a good and even coating of APTES a lot of papers...

02 March 2018 4,659 5 View

Can low concentrations in SERS have a larger intensity?

I have been synthesising Ag nanoparticle films on Silica beads, I wished to show the limit of detection by making a concentration serial dilution of Rhodamine 6 G, however, there was strange...

02 March 2018 7,893 3 View

How to remove aggregates from Microsphere preparations?

09 October 2016 1,676 3 View

Any suggestions on antibody pairs for a simple sandwich immunoassay?

I'm developing a dual mode detection immunoassay, based on antibody conjugated microspheres for the first method and fluorescent tagged antibodies for the second. What I'm looking for is any...

08 September 2016 9,976 3 View

Can DLS or a Zeta Sizer be used to get particle concentration?

We have magnetite spheres that are polystyrene coated and I wish to get analysis that tells me the concentration before and after magnetic recovery. Can the above mentioned techniques work?

01 February 2016 323 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View