What are the differences between stokes radius, radius of gyration and hydrodynamic radius ?? and how can they be measured (size exclusion chromatography, DLS, SAXS) ??
Strongly recommend that you look up each term on the web. That way you can learn about them and answer any questions that you may have. Learning how to research questions on your own is a critical skill that is best acquired as early as possible in your studies.
Hi, Mohammad.
I also know about this definition problem. As far as I know there is a big difference about the methods in use and the subject of interest. Rules of thumb often do not work out for proteins and most of the definitions were set up for Polymers. Typically, radius of gyration is obtained from Neutron scattering and hydrodynamic Radii are obtained from DLS. The Stokes Radius should be intrinsically related to the hydrodynamic Radius and the Radius of gyration Deals more with the inner Compactness of a molecule that also Depends on the axial Ratio of the shape. As William said, a thorough Literature survey would be the best choice. Then you will get a feeling for these values. Good luck.
Best, joerg
Thanks Mr. Simulescu for this clear explanation.
Can we perform DLS for any solution?? or it is just restricted to colloids or suspension (as nanoparticles)?
In other words, can we determine hydrodynamic radius for whatever solute?
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