I am looking for any studies that provide information on the potential (or lack thereof) of using the ice slurry method for anaesthesia in small fish.
I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...
01 July 2024 381 2 View
I have an antibody specific just for ICC and WB but I should use the same antibody to perform an IHC staining. Is it possible to use this ab also in IHC?
15 May 2024 6,392 3 View
Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...
10 April 2024 9,346 0 View
Title: Impact of Patents on Innovation in Language Technology Description: This research examines the influence of patents on innovation in language technology. It explores the balance between...
09 April 2024 9,061 1 View
How can we navigate the ethical challenges of employing AI in surveillance systems for cybercrime prevention, particularly concerning privacy rights? What design principles and safeguards should...
22 March 2024 5,267 3 View
Hi, I have to extract genomic DNA from plasma samples and will use the Qiamp DNA mini and blood kit (QIAGEN). At the moment the plasma samples are stored at -80° and they were obtained from...
04 March 2024 9,438 1 View
I have started to work with BV2 cells. First, I would like to condition the cells with DMEM 1X. The day after passing the cells, most had adhered to the flask surface; after another two days, most...
29 January 2024 4,450 0 View
Hi, I need to prepare stock solutions for Collagenase I from Sigma (C0130). The provider suggests to prepare solutions in TESCA buffer (50 mM TES, 0.36 mM CaCl2, pH 7.4 at 37 degrees). I tried to...
09 November 2023 2,239 2 View
Hello everyone, I'm dealing with some issues regarding resuspension and use of a Roche DNAse I that we purchased from Merck (Ref:10104159001). The product is purchased as a lyophilized vial (100...
08 November 2023 4,859 0 View
Hi everyone, I transduced my HEK cells with a viral vector containing mRuby as reporter, and now I would like to detect mRuby protein via Western Blot on my cells lysate. Apparently there is no...
05 October 2023 7,336 0 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...
23 July 2024 7,819 5 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,480 4 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
anyone looking for research collaboration on the anaerobic digestion slurry and digestate can contact me for further details. As my whatsapp number is +91 8317585217
11 July 2024 6,753 3 View
With many Northern Hemisphere countries approaching or exceeding the 95-95-95 criteria and with improved accessibility of community testing centers, I wonder whether we could significantly...
09 July 2024 2,181 1 View
I'm working on the proteome (from Uniprot), as you're probably aware that there are reviewed and unreviewed proteins, however unreviewed proteins are in large quantities, but reviewed proteins are...
09 July 2024 5,782 2 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View