I am currently seeking an immortalized bovine intestinal cell line for research purposes. If anyone has such a cell line available or knows someone who may be willing to share it, I would greatly appreciate your assistance.
Actually these are two separate questions: i. How to extract mycotoxins from the media PDA the fungal culture is growing on? ii. Can we extract the toxin from the natural media it is growing on?
06 September 2020 8,359 5 View
what to do after cause is known?
11 June 2020 4,191 0 View
Is there any inherent limitation in FDTD (as implemented by Remcom X-Fdtd or CST) that might preclude it from solving for certain electromagnetic wave modes? Specifically, I'm trying to simulate...
30 April 2019 3,271 1 View
I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...
17 February 2018 8,602 2 View
What are the tools available to measure decision making in the armed forces aspirants( preferably young adults)
26 November 2017 220 6 View
I work with micro satellite markers on nematodes. Would it be possible to generate genetic diversity and polymorphic content of the markers using excel? Or are there any simple ways to calculate...
24 September 2017 6,244 3 View
What are the methods available to do microdrilling of superalloys? The L/D ratio needs to be greater than 100. Any such facilities available in India?
14 July 2017 5,472 3 View
I need to find MoleculeInteraction energyVander Waals energyElectrostatic energyHydrogen bond energySolvation free energyInteracting energy I have already tried autodoc since the ligand is a...
10 July 2017 9,154 2 View
We are having trouble with loosing the top of some of our lanes on SDS gels. Any ideas why?
28 September 2016 6,903 6 View
Can I simply add a random sequence to the 5' end of my PCR primers and use this as sequence primers? The degenerate primers are designed against a highly conserved part of G-alpha subunits and...
16 May 2016 1,933 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View