Literature said it would be either 1:1 or 5:1 or some other ratios are also mentioned. what are the reason behind this ratio?
Zein Al-Abideen Douba
Mimicking physiological conditions: Lower ratios (e.g., 1:1) aim to replicate tumor microenvironments with limited immune infiltration, while higher ratios (e.g., 5:1 or 10:1) simulate conditions of strong T cell-mediated responses, as in immunotherapy. Assay sensitivity: Higher effector:target ratios improve detection of T cell cytotoxicity (e.g., via LDH release, flow cytometry, or IFN-γ secretion). T cell activation state: Activated T cells may kill more efficiently, allowing lower ratios to be effective. Experimental goal: Screening for antigen-specific responses may require lower ratios, while functional killing assays or drug testing may need higher ratios for clearer outcomes.
The choice of T cell:breast cancer cell ratio in co-culture experiments (e.g., 1:1, 5:1, or higher) is based on several biological and experimental factors:
Optimal ratios vary depending on the breast cancer cell line, T cell source (e.g., PBMCs vs. engineered T cells), and assay duration
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