Hi everyone,
I’m trying to extract DNA from a bacterial community culture using the FastDNA SPIN Kit for Soil. The extractions give me DNA, but I keep running into a problem: there’s a lot of salt carryover, which makes downstream steps like quantification and PCR quite messy.
The thing is, this is the kit we already have in the lab, and we’d like to make use of it before considering buying something new. So I’m wondering if anyone has found a way to reduce the salt contamination when using this kit — maybe with extra washes, buffer adjustments, or any other workaround — especially when the sample is not actually soil but a liquid culture.
Any suggestions would be really appreciated!