Does anyone have any experience regarding the estimation of ATP in muscle (meat) using a bioluminescence assay?
I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...
12 July 2024 8,116 2 View
for estimation of human health risks
26 June 2024 7,326 3 View
Trace amount of Cobalt-60 is generated in nuclear reactors and needs higher selective separation competing with sister heavy elements, especially Fe-triad. Cobalt has been seen to adsorb on...
28 May 2024 2,167 5 View
how to calculate the activation energy for grain and grain boundaries?
18 May 2024 5,413 0 View
This research question seeks to determine the average number of revision rounds a manuscript typically undergoes before being accepted by an SSCI journal. Understanding this average can help...
18 May 2024 5,815 6 View
(1) How to analyze relation between two variables in which data is obtained on the ordinal scale from two different groups and the data sets are asymmetric, where one group has significantly more...
15 May 2024 4,950 5 View
Hi, Could anyone tell me how to do to calculate optical density at 680 nm of microalgae culture when the suspension is diluted several times? Exemple: OD at 680 for a strain, in day 4 was 1.564...
20 March 2024 1,043 0 View
I am just curious to know what kind of chemical changes happen when ethanol comes in contact with materials made up of acrylic polymers, and how they crack.
01 March 2024 2,641 7 View
Hello everyone I am looking for the limitations on applying dynamic Bayesian networks (DBN); please provide scientific papers in this context.
19 February 2024 6,009 0 View
scale pdf, scoring, author's email to ask for permission.
13 January 2024 9,297 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View