For transformations Its ok to use acetosryingon but for cotyledons after gemination putting on Pre-Cultural medium is it necessary. can you suggests me.
Acetosryingon is added to infection and co-cultivation medium to improve transformation efficiency. Here, you did not mention clearly what are you doing? Are you doing transformation or just regeneration? If you put cotyledons on pre-culture medium then do transformation, I would recommend you to add AS to infection and co-cultivation medium.
I read your attached paper. Their plant is not tomato.
They used 100 mM AS to infect the cotyledon (they germinated the seeds to obtain the cotyledons, then, used the cotyledon as explants for infection in a 'transient assay' (see the yellow highlights, p409). I see nothing wrong with it.
Is this paper you indented to attach?
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