08 August 2016 1 2K Report

I extracted the total RNA from cyanobacteria and adding poly A using poly A polymerase, then synthesized cDNA using Thermo's kit. When using specific forward primer and oligo dT primer to amplify the target gene, I can't get any results. I wonder it's due to the annealing temprature (I used 55-45 ℃) or other reason?

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